More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| transferrin receptor | Polyclonal | IgG | Rabbit | Human | IHC-P, WB | Immunogen affinity purified. |
Immunogen | ||||||
| E.coli-derived human TFRC recombinant protein (Position: M1-N198). Human TFRC shares 72% and 70% amino acid (aa) sequences identity with mouse and rat TFRC, respectively. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | TFRC |
Protein | Transferrin receptor protein 1 |
Uniprot ID | P02786 |
Function | Cellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied transferrin receptor into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system (By similarity). A second ligand, the heditary hemochromatosis protein HFE, competes for binding with transferrin for an overlapping C-terminal binding site. |
Tissue Specificity | |
Sub-cellular localization | Cell membrane. |
Sequence Similarities | Belongs to the peptidase M28 family. M28B subfamily. |
Aliases | CD 71 antibody|CD71 antibody|CD71 antigen antibody|OTTHUMP00000208523 antibody|OTTHUMP00000208524 antibody|OTTHUMP00000208525 antibody|p90 antibody|sTfR antibody|T9 antibody|TFR 1 antibody|TFR antibody|TFR1 antibody|TFR1_HUMAN antibody|TFRC antibody|TR antibody|Transferrin receptor (p90 CD71) antibody|Transferrin Receptor Protein 1 antibody|Transferrin receptor protein 1, serum form antibody|TRFR antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti- TFRC antibody, ASA-B1849, Western blotting
All lanes: Anti TFRC (ASA-B1849) at 0.5ug/ml
WB: Recombinant Human TRFC Protein 0.5ng
Predicted bind size: 45KD
Observed bind size: 45KD
All lanes: Anti TFRC (ASA-B1849) at 0.5ug/ml
WB: Recombinant Human TRFC Protein 0.5ng
Predicted bind size: 45KD
Observed bind size: 45KD
Anti- TFRC antibody, ASA-B1849, Western blotting
All lanes: Anti TRFC (ASA-B1849) at 0.5ug/ml
Lane 1: HELA Whole Cell Lysate at 40ug
Lane 2: JURAKT Whole Cell Lysate at 40ug
Lane 3: RAJI Whole Cell Lysate at 40ug
Lane 4: HL-60 Whole Cell Lysate at 40ug
Lane 5: K562 Whole Cell Lysate at 40ug
Lane 6: HEPG2 Whole Cell Lysate at 40ug
Lane 7: Human Placenta Tissue Lysate at 50ug
Lane 8: CEM Whole Cell Lysate at 40ug
Predicted bind size: 86KD
Observed bind si
All lanes: Anti TRFC (ASA-B1849) at 0.5ug/ml
Lane 1: HELA Whole Cell Lysate at 40ug
Lane 2: JURAKT Whole Cell Lysate at 40ug
Lane 3: RAJI Whole Cell Lysate at 40ug
Lane 4: HL-60 Whole Cell Lysate at 40ug
Lane 5: K562 Whole Cell Lysate at 40ug
Lane 6: HEPG2 Whole Cell Lysate at 40ug
Lane 7: Human Placenta Tissue Lysate at 50ug
Lane 8: CEM Whole Cell Lysate at 40ug
Predicted bind size: 86KD
Observed bind si
Anti- TFRC antibody, ASA-B1849,IHC(P)
IHC(P): Human Placenta Tissue
IHC(P): Human Placenta Tissue