More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| tissue factor pathway inhibitor(lipoprotein-associated coagulation inhibitor) | Polyclonal | IgG | Rabbit | Human | IHC-P, WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the N-terminus of human TFPI(261-280aa QECLRACKKGFIQRISKGGL). | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | TFPI |
Protein | Tissue factor pathway inhibitor |
Uniprot ID | P10646 |
Function | Inhibits factor X (X(a)) directly and, in a Xa-dependent way, inhibits VIIa/tissue factor activity, presumably by forming a quaternary Xa/LACI/VIIa/TF complex. It possesses an antithrombotic action and also the ability to associate with lipoproteins in plasma. |
Tissue Specificity | Mostly in endothelial cells. |
Sub-cellular localization | Isoform Alpha: Secreted. |
Sequence Similarities | Contains 3 BPTI/Kunitz inhibitor domains. |
Aliases | Anti convertin antibody|EPI antibody|Extrinsic pathway inhibitor antibody|LACI antibody|Lipoprotein associated coagulation inhibitor antibody|Lipoprotein-associated coagulation inhibitor antibody|TFI antibody|TFPI 1 antibody|TFPI antibody|TFPI1 antibody|TFPI1_HUMAN antibody|Tissue factor pathway inhibitor(lipoprotein associated coagulation inhibitor) antibody|Tissue factor pathway inhibitor antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti- TFPI antibody, ASA-B1847, Western blotting
All lanes: Anti TFPI (ASA-B1847) at 0.5ug/ml
WB: SMMC Whole Cell Lysate at 40ug
Predicted bind size: 26KD
Observed bind size: 55KD
All lanes: Anti TFPI (ASA-B1847) at 0.5ug/ml
WB: SMMC Whole Cell Lysate at 40ug
Predicted bind size: 26KD
Observed bind size: 55KD
Anti- TFPI antibody, ASA-B1847, IHC(P)
IHC(P): Human Placenta Tissue
IHC(P): Human Placenta Tissue
Anti- TFPI antibody, ASA-B1847, IHC(P)
IHC(P): Human Lung Cancer Tissue
IHC(P): Human Lung Cancer Tissue