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Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| tenascin C | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | IHC-P, WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of mouse Tenascin C(2021-2036aa DSMNYHNGRSFSTYDK), identical to the related rat sequence, and different from the related human sequence by two amino acids. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | TNC |
Protein | Tenascin |
Uniprot ID | Q80YX1 |
Function | Extracellular matrix protein implicated in guidance of migrating neurons as well as axons during development, synaptic plasticity as well as neuronal regeneration. Promotes neurite outgrowth when provided to neurons in culture. May play a role in supporting the growth of epithelial tumors. Ligand for integrins alpha-8/beta-1, alpha-9/beta-1, alpha-V/beta-3 and alpha-V/beta-6. |
Tissue Specificity | High levels of isoform 2 in lung and brain of newborn mice. High levels of isoform 5 in thymus, moderate levels in brain of newborn and adult mice. Low level of isoform 2 in adult brain. |
Sub-cellular localization | Secreted, extracellular space, extracellular matrix . |
Sequence Similarities | Belongs to the tenascin family. |
Aliases | 150 225 antibody|Cytotactin antibody|Glioma associated extracellular matrix antigen antibody|Glioma-associated-extracellular matrix antigen antibody|GMEM antibody|GP 150 225 antibody|GP 150-225 antibody|GP antibody|Hexabrachion antibody|HXB antibody|JI antibody|MGC167029 antibody|Miotendinous antigen antibody|Myotendinous antigen antibody|Neuronectin antibody|TENA_HUMAN antibody|Tenascin antibody|Tenascin-C antibody|Tenascin-C isoform 14/AD1/16 antibody|TenascinC antibody|TN antibody|TN C antibody|TN-C antibody|TNC antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Rat Mouse | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human, Rat Mouse | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti-Tenascin C antibody, ASA-B1845, Western blotting
Lane 1: Human Placenta Tissue Lysate
Lane 2: Rat Brain Tissue Lysate
Lane 1: Human Placenta Tissue Lysate
Lane 2: Rat Brain Tissue Lysate
Anti-Tenascin C antibody, ASA-B1845, IHC(P)
IHC(P): Human Placenta Tissue
IHC(P): Human Placenta Tissue
Anti-Tenascin C antibody, ASA-B1845, IHC(P)
IHC(P): Rat Cardiac Muscle Tissue
IHC(P): Rat Cardiac Muscle Tissue