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Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| syndecan 4 | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | IHC-P, WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of human Syndecan 4 (172-198aa MKKKDEGSYDLGKKPIYKKAPTNEFYA), identical to the related mouse and rat sequences. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | SDC4 |
Protein | Syndecan-4 |
Uniprot ID | P31431 |
Function | Cell surface proteoglycan that bears heparan sulfate. |
Tissue Specificity | Expressed in epithelial and fibroblastic cells. |
Sub-cellular localization | Isoform 1: Membrane; Single-pass type I membrane protein. Secreted. Note: Shedding of the ectodomain produces a soluble form. |
Sequence Similarities | |
Aliases | Amphiglycan antibody|Amphiglycan antibody|MGC22217 antibody| OTTHUMP00000031788 antibody| ryudocan amphiglycan antibody|Ryudocan antibody|Ryudocan core protein antibody|Ryudocan core protein antibody|SDC 4 antibody|SDC4 antibody|SDC4_HUMAN antibody|SYND 4 antibody| SYND4 antibody|syndecan 4 antibody|syndecan 4 (amphiglycan, ryudocan) antibody| syndecan proteoglycan 4 antibody|Syndecan-4 antibody|Syndecan4 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Rat | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human, Mouse, Rat | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti- Syndecan 4 antibody, ASA-B1824, Western blotting
All lanes: Anti Syndecan 4 (ASA-B1824) at 0.5ug/ml
Lane 1: MCF-7 Whole Cell Lysate at 40ug
Lane 2: SW620 Whole Cell Lysate at 40ug
Lane 3: Rat Lung Tissue Lysate at 50ug
Predicted bind size: 22KD
Observed bind size: 22KD
All lanes: Anti Syndecan 4 (ASA-B1824) at 0.5ug/ml
Lane 1: MCF-7 Whole Cell Lysate at 40ug
Lane 2: SW620 Whole Cell Lysate at 40ug
Lane 3: Rat Lung Tissue Lysate at 50ug
Predicted bind size: 22KD
Observed bind size: 22KD
Anti- Syndecan 4 antibody, ASA-B1824, IHC(P)
IHC(P): Mouse Intestine Tissue
IHC(P): Mouse Intestine Tissue
Anti- Syndecan 4 antibody, ASA-B1824, IHC(P)
IHC(P): Rat Intestine Tissue
IHC(P): Rat Intestine Tissue