Syndecan 4  Antibody View larger

Syndecan 4 Antibody

ASA-B1824

$365.00

More info

Overview

Long Name

Antibody Type

Antibody Isotype

Host

Species Reactivity

Validated Applications

Purification 

syndecan 4 Polyclonal IgG Rabbit Human, Mouse, Rat IHC-P, WB Immunogen affinity purified.

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human Syndecan 4 (172-198aa MKKKDEGSYDLGKKPIYKKAPTNEFYA), identical to the related mouse and rat sequences.

Properties

Form

Lyophilized

Size

100 µg/vial

Contents

Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request.

Concentration

Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration).

Storage

At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing.

Additional Information Regarding the Antigen

Gene

SDC4

Protein

Syndecan-4

Uniprot ID

P31431

Function

Cell surface proteoglycan that bears heparan sulfate.

Tissue Specificity

Expressed in epithelial and fibroblastic cells.

Sub-cellular localization

Isoform 1: Membrane; Single-pass type I membrane protein. Secreted. Note: Shedding of the ectodomain produces a soluble form.

Sequence Similarities

Aliases

Amphiglycan antibody|Amphiglycan antibody|MGC22217 antibody| OTTHUMP00000031788 antibody| ryudocan amphiglycan antibody|Ryudocan antibody|Ryudocan core protein antibody|Ryudocan core protein antibody|SDC 4 antibody|SDC4 antibody|SDC4_HUMAN antibody|SYND 4 antibody| SYND4 antibody|syndecan 4 antibody|syndecan 4 (amphiglycan, ryudocan) antibody| syndecan proteoglycan 4 antibody|Syndecan-4 antibody|Syndecan4 antibody

Application Details

Application Concentration* Species Validated Using**
Western blot 0.1-0.5μg/ml Human, Rat AssaySolutio's ECL kit
Immunohistochemistry(Paraffin-embedded Section) 0.5-1μg/ml Human, Mouse, RatAssaySolutio's IHC/ICC Detection kit

AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti- Syndecan 4 antibody, ASA-B1824, Western blotting
All lanes: Anti Syndecan 4 (ASA-B1824) at 0.5ug/ml
Lane 1: MCF-7 Whole Cell Lysate at 40ug
Lane 2: SW620 Whole Cell Lysate at 40ug
Lane 3: Rat Lung Tissue Lysate at 50ug
Predicted bind size: 22KD
Observed bind size: 22KD
Anti- Syndecan 4 antibody, ASA-B1824, IHC(P)
IHC(P): Mouse Intestine Tissue
Anti- Syndecan 4 antibody, ASA-B1824, IHC(P)
IHC(P): Rat Intestine Tissue

© 2024 Novateinbio.com