More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| synaptopodin | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | IHC-P, WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence in the middle region of human Synaptopodin(495-509aa TSQMERSPMLERRHF), identical to the related mouse and rat sequences. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | SYNPO |
Protein | Synaptopodin(SYNPO) |
Uniprot ID | Q8N3V7 |
Function | Actin-associated protein that may play a role in modulating actin-based shape and motility of dendritic spines and renal podocyte foot processes. Seems to be essential for the formation of spine apparatuses in spines of telencephalic neurons, which is involved in synaptic plasticity (By similarity). |
Tissue Specificity | Expressed in cerebral cortex. |
Sub-cellular localization | Cytoplasm, cytoskeleton . Cell junction, tight junction . Perikaryon . Cell projection, dendritic spine . Cell junction, synapse, postsynaptic cell membrane, postsynaptic density . Cell junction, synapse . Note: Localized at the tight junction of cells. In brain, localized to the postsynaptic densities and in the perikarya. Associated with dendritic spines of a subset of synapses (By similarity). |
Sequence Similarities | Belongs to the synaptopodin family. |
Aliases | KIAA1029 antibody|Synaptopodin antibody|SYNPO antibody|SYNPO_HUMAN antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Rat Human, Mouse | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human, Rat Mouse | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti-Synaptopodin antibody, ASA-B1818, Western blotting
All lanes: Anti Synaptopodin (ASA-B1818) at 0.5ug/ml
WB: Rat Brain Tissue Lysate at 50ug
Predicted bind size: 99KD
Observed bind size: 99KD
All lanes: Anti Synaptopodin (ASA-B1818) at 0.5ug/ml
WB: Rat Brain Tissue Lysate at 50ug
Predicted bind size: 99KD
Observed bind size: 99KD
