More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| surfactant protein D | Polyclonal | IgG | Rabbit | Human, Rat | ELISA, IHC-P, WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of human Surfactant protein D (292-321aa RSAAENAALQQLVVAKNEAAFLSMTD SKTE), different from the related mouse and rat sequences by eight amino acids. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | SFTPD |
Protein | Pulmonary surfactant-associated protein D |
Uniprot ID | P35247 |
Function | Contributes to the lung's defense against inhaled microorganisms, organic antigens and toxins. Interacts with compounds such as bacterial lipopolysaccharides, oligosaccharides and fatty acids and modulates leukocyte action in immune response. May participate in the extracellular reorganization or turnover of pulmonary surfactant. Binds strongly maltose residues and to a lesser extent other alpha-glucosyl moieties. |
Tissue Specificity | Expressed in lung, brain, pancreas and adipose tissue (mainly mature adipocytes). |
Sub-cellular localization | Secreted, extracellular space, extracellular matrix. Secreted, extracellular space, surface film. |
Sequence Similarities | Belongs to the SFTPD family. |
Aliases | COLEC 7 antibody|COLEC7 antibody|Collectin-7 antibody|Collectin7 antibody|Lung surfactant protein D antibody|PSP D antibody|PSP-D antibody|PSP-D Surfactant protein D antibody|PSPD antibody|Pulmonary surfactant apoprotein antibody|Pulmonary surfactant associated protein D antibody|Pulmonary surfactant associated protein PSP-D antibody|Pulmonary surfactant-associated protein D antibody|SFTP 4 antibody|SFTP4 antibody|SFTPD antibody|SFTPD_HUMAN antibody|SP D antibody|SP-D antibody|Surfactant associated protein pulmonary 4 antibody|Surfactant pulmonary associated protein D antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Rat | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human | AssaySolutio's IHC/ICC Detection kit |
| ELISA | 0.1-0.5μg/ml | Human | Sandwich ELISA format |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti- Surfactant protein D antibody, ASA-B1814, Western blotting
All lanes: Anti Surfactant protein D (ASA-B1814) at 0.5ug/ml
Lane 1: Rat Lung Tissue Lysate at 50ug
Lane 2: Rat Brain Tissue Lysate at 50ug
Lane 3: PANC Whole Cell Lysate at 40ug
Predicted bind size: 38KD
Observed bind size: 38KD
All lanes: Anti Surfactant protein D (ASA-B1814) at 0.5ug/ml
Lane 1: Rat Lung Tissue Lysate at 50ug
Lane 2: Rat Brain Tissue Lysate at 50ug
Lane 3: PANC Whole Cell Lysate at 40ug
Predicted bind size: 38KD
Observed bind size: 38KD
Anti- Surfactant protein D antibody, ASA-B1814, IHC(P)
IHC(P): Human Lung Cancer Tissue
IHC(P): Human Lung Cancer Tissue
