More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| cystatin B (stefin B) | Polyclonal | IgG | Rabbit | Human | ELISA, IHC-P, WB | Immunogen affinity purified. |
Immunogen | ||||||
| E. coli-derived human Stefin B recombinant protein (Position: M1-F98). Human Stefin B shares 78.6 % amino acid (aa) sequence identity with both mouse and rat Stefin B. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | CSTB |
Protein | Cystatin-B |
Uniprot ID | P04080 |
Function | This is an intracellular thiol proteinase inhibitor. Tightly binding reversible inhibitor of cathepsins L, H and B. |
Tissue Specificity | |
Sub-cellular localization | Cytoplasm. |
Sequence Similarities | Belongs to the cystatin family. |
Aliases | CHROW21 antibody|CPI B antibody|CPI-B antibody|CST6 antibody|CSTB antibody|Cystatin B antibody|Cystatin-B antibody|CYTB antibody|CYTB_HUMAN antibody|EPM1 antibody|EPM1A antibody|Liver thiol proteinase inhibitor antibody| PME antibody|Stefin-B antibody|STFB antibody|ULD antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human | AssaySolutio's IHC/ICC Detection kit |
| ELISA | 0.1-0.5μg/ml | Human | Sandwich ELISA format |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti- Stefin B antibody, ASA-B1806, Western blotting
All lanes: Anti Stefin B (ASA-B1806) at 0.5ug/ml
Lane 1: Human Placenta Tissue Lysate at 50ug
Lane 2: HELA Whole Cell Lysate at 40ug
Lane 3: A431 Whole Cell Lysate at 40ug
Lane 4: A549 Whole Cell Lysate at 40ug
Predicted bind size: 11KD
Observed bind size: 11KD
All lanes: Anti Stefin B (ASA-B1806) at 0.5ug/ml
Lane 1: Human Placenta Tissue Lysate at 50ug
Lane 2: HELA Whole Cell Lysate at 40ug
Lane 3: A431 Whole Cell Lysate at 40ug
Lane 4: A549 Whole Cell Lysate at 40ug
Predicted bind size: 11KD
Observed bind size: 11KD
Anti- Stefin B antibody, ASA-B1806,IHC(P)
IHC(P): Human Prostatic Cancer Tissue
IHC(P): Human Prostatic Cancer Tissue
