More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| synovial sarcoma, X breakpoint 2 | Polyclonal | IgG | Rabbit | Human | IHC-P, WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of human Protein SSX2(157-184aa NREAQEKEERRGTAHRWSSQNTHNIGRF). | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | Protein SSX2 |
Protein | SSX2 |
Uniprot ID | Q16385 |
Function | Could act as a modulator of transcription. |
Tissue Specificity | Expressed at high level in the testis. Expressed at low level in thyroid. Not detected in tonsil, colon, lung, spleen, prostate, kidney, striated and smooth muscles. Detected in rhabdomyosarcoma and fibrosarcoma cell lines. Not detected in mesenchymal and epithelial cell lines. |
Sub-cellular localization | Nucleus. |
Sequence Similarities | Belongs to the SSX family. |
Aliases | Cancer/testis antigen 5.2 antibody|CT5.2 antibody|HD21 antibody|hom mel 40 antibody|HOM-MEL-40 antibody|MGC119055 antibody|MGC15364 antibody|MGC3884 antibody|Protein SSX2 antibody|RP11-552J9.2 antibody|sarcoma, synovial, X-chromosome related 2 antibody|SSx antibody|SSX2 antibody|SSX2-SYT fusion gene, included antibody|SSX2_HUMAN antibody|SSX2B antibody|Synovial sarcoma antibody|synovial sarcoma X breakpoint 2 antibody|Tumor antigen HOM-MEL-40 antibody|X breakpoint 2 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti-SSX2 antibody, ASA-B1785, Western blotting
Lane 1: MCF-7 Cell Lysate
Lane 2: HELA Cell Lysate
Lane 1: MCF-7 Cell Lysate
Lane 2: HELA Cell Lysate
Anti-SSX2 antibody, ASA-B1785, IHC(P)
IHC(P): Human Intestinal Cancer Tissue
IHC(P): Human Intestinal Cancer Tissue
