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Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| abl-interactor 1 | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | IHC-P, WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the N-terminus of human SSH3BP1 (151-175aa HGVKWLKAKHGNNQPARTGTLSRTN), identical to the related mouse and rat sequences. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | ABI1 |
Protein | Abl interactor 1 |
Uniprot ID | Q8IZP0 |
Function | May act in negative regulation of cell growth and transformation by interacting with nonreceptor tyrosine kinases ABL1 and/or ABL2. May play a role in regulation of EGF-induced Erk pathway activation. Involved in cytoskeletal reorganization and EGFR signaling. Together with EPS8 participates in transduction of signals from Ras to Rac. In vitro, a trimeric complex of ABI1, EPS8 and SOS1 exhibits Rac specific guanine nucleotide exchange factor (GEF) activity and ABI1 seems to act as an adapter in the complex. Regulates ABL1/c-Abl-mediated phosphorylation of ENAH. Recruits WASF1 to lamellipodia and there seems to regulate WASF1 protein level. In brain, seems to regulate the dendritic outgrowth and branching as well as to determine the shape and number of synaptic contacts of developing neurons. |
Tissue Specificity | Widely expressed, with highest expression in brain. |
Sub-cellular localization | Cytoplasm . Nucleus . Cell projection, lamellipodium . Cell projection, filopodium . Cell projection, growth cone . Cell junction, synapse, postsynaptic cell membrane, postsynaptic density . Cytoplasm, cytoskeleton . Note: Localized to protruding lamellipodia and filopodia tips. Also localized to neuronal growth cones and synaptosomes. May shuttle from the postsynaptic densities to the nucleus (By similarity). |
Sequence Similarities | Belongs to the ABI family. |
Aliases | Abelson interactor 1 antibody|ABI 1 antibody|Abi-1 antibody|ABI1 antibody| ABI1_HUMAN antibody|Abl binding protein 4 antibody|Abl interactor 1 antibody|Abl interactor protein 1 antibody|Abl interactor protein 1 long antibody|Abl-binding protein 4 antibody|AblBP4 antibody|Ablphilin 1 antibody|E3B1 antibody|Eps8 binding protein antibody|Eps8 SH3 domain binding protein antibody|Eps8 SH3 domain-binding protein antibody|Eps8-binding protein antibody|Hssh3bp1 antibody|Interactor protein AblBP4 antibody|NAP1 antibody|Nap1 binding protein antibody|Nap1-binding protein antibody|NAP1BP antibody|Spectrin SH3 domain binding protein 1 antibody|Spectrin SH3 domain-binding protein 1 antibody| SSH3BP antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Mouse, Rat | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Mouse, Rat Human | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti- SSH3BP1 antibody, ASA-B1783, Western blotting
All lanes: Anti SSH3BP1 (ASA-B1783) at 0.5ug/ml
Lane 1: Rat Liver Tissue Lysate at 50ug
Lane 2: Rat Testis Tissue Lysate at 50ug
Lane 3: HELA Whole Cell Lysate at 40ug
Lane 4: RH35 Whole Cell Lysate at 40ug
Lane 5: HEPA Whole Cell Lysate at 40ug
Predicted bind size: 55KD
Observed bind size: 55KD
All lanes: Anti SSH3BP1 (ASA-B1783) at 0.5ug/ml
Lane 1: Rat Liver Tissue Lysate at 50ug
Lane 2: Rat Testis Tissue Lysate at 50ug
Lane 3: HELA Whole Cell Lysate at 40ug
Lane 4: RH35 Whole Cell Lysate at 40ug
Lane 5: HEPA Whole Cell Lysate at 40ug
Predicted bind size: 55KD
Observed bind size: 55KD
Anti- SSH3BP1 antibody, ASA-B1783, IHC(P)
IHC(P): Mouse Brain Tissue
IHC(P): Mouse Brain Tissue
Anti- SSH3BP1 antibody, ASA-B1783, IHC(P)
IHC(P): Rat Brain Tissue
IHC(P): Rat Brain Tissue