SQSTM1/p62 Antibody View larger

SQSTM1/p62 Antibody

ASA-B1777

$330.00

More info

Overview

Long Name

Antibody Type

Antibody Isotype

Host

Species Reactivity

Validated Applications

Purification 

sequestosome 1 Polyclonal IgG Rabbit Human, Mouse, Rat IHC-P, ICC, WB Immunogen affinity purified.

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminus of human SQSTM1(91-110aa KDDIFRIYIKEKKECRRDHR), different from the related rat and mouse sequences by one amino acid.

Properties

Form

Lyophilized

Size

100 µg/vial

Contents

Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request.

Concentration

Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration).

Storage

At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing.

Additional Information Regarding the Antigen

Gene

SQSTM1

Protein

Sequestosome-1

Uniprot ID

Q13501

Function

Autophagy receptor that interacts directly with both the cargo to become degraded and an autophagy modifier of the MAP1 LC3 family. Required both for the formation and autophagic degradation of polyubiquitin-containing bodies, called ALIS (aggresome-like induced structures) and links ALIS to the autophagic machinery. Involved in midbody ring degradation. May regulate the activation of NFKB1 by TNF-alpha, nerve growth factor (NGF) and interleukin- 1. May play a role in titin/TTN downstream signaling in muscle cells. May regulate signaling cascades through ubiquitination. Adapter that mediates the interaction between TRAF6 and CYLD (By similarity). May be involved in cell differentiation, apoptosis, immune response and regulation of K(+) channels.

Tissue Specificity

Ubiquitously expressed.

Sub-cellular localization

Cytoplasm. Late endosome. Lysosome. Cytoplasmic vesicle, autophagosome. Nucleus. Endoplasmic reticulum. Cytoplasm, P-body. Note: Sarcomere (By similarity). In cardiac muscles localizes to the sarcomeric band (By similarity). Commonly found in inclusion bodies containing polyubiquitinated protein aggregates. In neurodegenerative diseases, detected in Lewy bodies in Parkinson disease, neurofibrillary tangles in Alzheimer disease, and HTT aggregates in Huntington disease. In protein aggregate diseases of the liver, found in large amounts in Mallory bodies of alcoholic and nonalcoholic steatohepatitis, hyaline bodies in hepatocellular carcinoma, and in SERPINA1 aggregates. Enriched in Rosenthal fibers of pilocytic astrocytoma. In the cytoplasm, observed in both membrane-free ubiquitin- containing protein aggregates (sequestosomes) and membrane- surrounded autophagosomes. Colocalizes with TRIM13 in the perinuclear endoplasmic reticulum. Co-localizes with TRIM5 in the cytoplasmic bodies.

Sequence Similarities

Contains 1 PB1 domain.

Aliases

A170 antibody|EBI 3 associated protein of 60 kDa antibody|EBI 3 associated protein p60 antibody|EBI3 associated protein of 60 kDa antibody|EBI3 associated protein p60 antibody|EBI3-associated protein of 60 kDa antibody|EBIAP antibody|MGC127197 antibody|ORCA antibody|OSF-6 antibody|Osi antibody|OSIL antibody|Oxidative stress induced like antibody|p60 antibody|p62 antibody|p62B antibody|Paget disease of bone 3 antibody|PDB 3 antibody|PDB3 antibody|Phosphotyrosine independent ligand for the Lck SH2 domain of 62 kDa antibody|Phosphotyrosine independent ligand for the Lck SH2 domain p62 antibody|Phosphotyrosine-independent ligand for the Lck SH2 domain of 62 kDa antibody|PKC-zeta-interacting protein antibody|Protein kinase C-zeta-interacting protein antibody|Sequestosome 1 antibody|Sequestosome-1 antibody|SQSTM 1 antibody|SQSTM_HUMAN antibody|Sqstm1 antibody|STAP antibody|STONE14 antibody|Ubiquitin binding protein p62 antibody|Ubiquitin-binding protein p62 antibody|ZIP 3 antibody|ZIP antibody|ZIP3 antibody

Application Details

Application Concentration* Species Validated Using**
Western blot 0.1-0.5μg/ml Human, Rat MouseAssaySolutio's ECL kit
Immunohistochemistry(Paraffin-embedded Section) 0.5-1μg/ml Human, Rat MouseAssaySolutio's IHC/ICC Detection kit
Immunocytochemistry 0.5-1μg/ml Human Mouse, RatAssaySolutio's IHC/ICC Detection kit

AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P) and ICC. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-SQSTM1/p62 antibody, ASA-B1777, Western blotting
Lane 1: Rat Brain Tissue Lysate
Lane 2: HELA Cell Lysate
Lane 3: U87 Cell Lysate
Lane 4: A549 Cell Lysate
Anti-SQSTM1/p62 antibody, ASA-B1777, IHC(P)
IHC(P): Human Intestinal Cancer Tissue
Anti-SQSTM1/p62 antibody, ASA-B1777, ICC
ICC: Hela Cell

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