More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| sequestosome 1 | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | IHC-P, IHC-F, WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the N-terminus of human SQSTM1/p62 (69-96aa DEDGDLVAFSSDEELTMAMSYVKDDIFR), identical to the related mouse and rat sequences. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | SQSTM1 |
Protein | Sequestosome-1 |
Uniprot ID | Q13501 |
Function | Autophagy receptor that interacts directly with both the cargo to become degraded and an autophagy modifier of the MAP1 LC3 family. Required both for the formation and autophagic degradation of polyubiquitin-containing bodies, called ALIS (aggresome-like induced structures) and links ALIS to the autophagic machinery. Involved in midbody ring degradation. May regulate the activation of NFKB1 by TNF-alpha, nerve growth factor (NGF) and interleukin- 1. May play a role in titin/TTN downstream signaling in muscle cells. May regulate signaling cascades through ubiquitination. Adapter that mediates the interaction between TRAF6 and CYLD (By similarity). May be involved in cell differentiation, apoptosis, immune response and regulation of K(+) channels. |
Tissue Specificity | Ubiquitously expressed. |
Sub-cellular localization | Cytoplasm. Late endosome. Lysosome. Cytoplasmic vesicle, autophagosome. Nucleus. Endoplasmic reticulum. Cytoplasm, P-body. Note: Sarcomere (By similarity). In cardiac muscles localizes to the sarcomeric band (By similarity). Commonly found in inclusion bodies containing polyubiquitinated protein aggregates. In neurodegenerative diseases, detected in Lewy bodies in Parkinson disease, neurofibrillary tangles in Alzheimer disease, and HTT aggregates in Huntington disease. In protein aggregate diseases of the liver, found in large amounts in Mallory bodies of alcoholic and nonalcoholic steatohepatitis, hyaline bodies in hepatocellular carcinoma, and in SERPINA1 aggregates. Enriched in Rosenthal fibers of pilocytic astrocytoma. In the cytoplasm, observed in both membrane-free ubiquitin- containing protein aggregates (sequestosomes) and membrane- surrounded autophagosomes. Colocalizes with TRIM13 in the perinuclear endoplasmic reticulum. Co-localizes with TRIM5 in the cytoplasmic bodies. |
Sequence Similarities | Contains 1 PB1 domain. |
Aliases | A170 antibody|EBI 3 associated protein of 60 kDa antibody|EBI 3 associated protein p60 antibody|EBI3 associated protein of 60 kDa antibody|EBI3 associated protein p60 antibody| EBI3-associated protein of 60 kDa antibody| EBIAP antibody|MGC127197 antibody|ORCA antibody| OSF-6 antibody|Osi antibody|OSIL antibody|Oxidative stress induced like antibody|p60 antibody| p62 antibody|p62B antibody|Paget disease of bone 3 antibody|PDB 3 antibody| PDB3 antibody|Phosphotyrosine independent ligand for the Lck SH2 domain of 62 kDa antibody| Phosphotyrosine independent ligand for the Lck SH2 domain p62 antibody|Phosphotyrosine-independent ligand for the Lck SH2 domain of 62 kDa antibody|PKC-zeta-interacting protein antibody|Protein kinase C-zeta-interacting protein antibody|Sequestosome 1 antibody| Sequestosome-1 antibody|SQSTM 1 antibody|SQSTM_HUMAN antibody| Sqstm1 antibody|STAP antibody|STONE14 antibody|Ubiquitin binding protein p62 antibody|Ubiquitin-binding protein p62 antibody|ZIP 3 antibody|ZIP antibody|ZIP3 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human, Mouse, Rat | AssaySolutio's IHC/ICC Detection kit |
| Immunohistochemistry(Frozen Section) | 0.5-1μg/ml | Mouse, Rat | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P) and IHC(F). *Blocking peptide can be purchased at $65. Contact us for more information
Anti- SQSTM1 antibody, ASA-B1778, Western blotting
All lanes: Anti SQSTM1 (ASA-B1778) at 0.5ug/ml
Lane 1: HELA Whole Cell Lysate at 40ug
Lane 2: JURKAT Whole Cell Lysate at 40ug
Lane 3: A549 Whole Cell Lysate at 40ug
Predicted bind size: 48KD
Observed bind size: 48KD
All lanes: Anti SQSTM1 (ASA-B1778) at 0.5ug/ml
Lane 1: HELA Whole Cell Lysate at 40ug
Lane 2: JURKAT Whole Cell Lysate at 40ug
Lane 3: A549 Whole Cell Lysate at 40ug
Predicted bind size: 48KD
Observed bind size: 48KD
Anti- SQSTM1 antibody, ASA-B1778,IHC(P)
IHC(P): Mouse Thymus Tissue
IHC(P): Mouse Thymus Tissue
Anti- SQSTM1 antibody, ASA-B1778,IHC(P)
IHC(P): Rat Spleen Tissue
IHC(P): Rat Spleen Tissue