More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| serine palmitoyltransferase, long chain base subunit 1 | Polyclonal | IgG | Rabbit | Human | IHC-P, WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence in the middle region of human SPTLC1(138-154aa FYGTFDVHLDLEDRLAK). | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | SPTLC1 |
Protein | Serine palmitoyltransferase 1 |
Uniprot ID | O15269 |
Function | Serine palmitoyltransferase (SPT). The heterodimer formed with SPTLC2 or SPTLC3 constitutes the catalytic core. The composition of the serine palmitoyltransferase (SPT) complex determines the substrate preference. The SPTLC1-SPTLC2-SPTSSA complex shows a strong preference for C16-CoA substrate, while the SPTLC1-SPTLC3-SPTSSA isozyme uses both C14-CoA and C16-CoA as substrates, with a slight preference for C14-CoA. The SPTLC1- SPTLC2-SPTSSB complex shows a strong preference for C18-CoA substrate, while the SPTLC1-SPTLC3-SPTSSB isozyme displays an ability to use a broader range of acyl-CoAs, without apparent preference. |
Tissue Specificity | Widely expressed. Not detected in small intestine. |
Sub-cellular localization | Endoplasmic reticulum membrane ; Single-pass membrane protein . |
Sequence Similarities | Belongs to the class-II pyridoxal-phosphate-dependent aminotransferase family. |
Aliases | HSAN antibody|HSAN1 antibody|HSN1 antibody|LBC1 antibody|LCB 1 antibody|LCB1 antibody|Long chain base biosynthesis protein 1 antibody|MGC14645 antibody|Serine C palmitoyltransferase antibody|Serine palmitoyl CoA transferase 1 antibody|Serine palmitoyltransferase 1 antibody|Serine palmitoyltransferase long chain base subunit 1 antibody|Serine palmitoyltransferase subunit 1 antibody|Serine-palmitoyl-CoA transferase 1 antibody|SPT 1 antibody|SPT1 antibody|SPTC1_HUMAN antibody|SPTI antibody|SPTLC 1 antibody|SPTLC1 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti-SPTLC1 antibody, ASA-B1776, Western blotting
All lanes: Anti SPTLC1 (ASA-B1776) at 0.5ug/ml
Lane 1: SW620 Whole Cell Lysate at 40ug
Lane 2: S549 Whole Cell Lysate at 40ug
Lane 3: PANC Whole Cell Lysate at 40ug
Lane 4: U87 Whole Cell Lysate at 40ug
Predicted bind size: 53KD
Observed bind size: 53KD
All lanes: Anti SPTLC1 (ASA-B1776) at 0.5ug/ml
Lane 1: SW620 Whole Cell Lysate at 40ug
Lane 2: S549 Whole Cell Lysate at 40ug
Lane 3: PANC Whole Cell Lysate at 40ug
Lane 4: U87 Whole Cell Lysate at 40ug
Predicted bind size: 53KD
Observed bind size: 53KD
Anti-SPTLC1 antibody, ASA-B1776, IHC(P)
IHC(P): Human Mammary Cancer Tissue
IHC(P): Human Mammary Cancer Tissue
