More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| sphingosine kinase 1 | Polyclonal | IgG | Rabbit | Human, Mouse | IHC-P, ICC, WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence in the middle region of human SPHK1(117-133aa AASLNHYAGYEQVTNED), different from the related rat sequence by two amino acids, and from the related mouse sequence by one amino acid. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | SPHK1 |
Protein | Sphingosine kinase 1 |
Uniprot ID | Q9NYA1 |
Function | Catalyzes the phosphorylation of sphingosine to form sphingosine 1-phosphate (SPP), a lipid mediator with both intra- and extracellular functions. Also acts on D-erythro-sphingosine and to a lesser extent sphinganine, but not other lipids, such as D,L-threo-dihydrosphingosine, N,N-dimethylsphingosine, diacylglycerol, ceramide, or phosphatidylinositol. |
Tissue Specificity | Widely expressed with highest levels in adult liver, kidney, heart and skeletal muscle. |
Sub-cellular localization | Cytoplasm. Nucleus. Cell membrane. Note: Translocated from the cytoplasm to the plasma membrane in a CIB1-dependent manner. |
Sequence Similarities | |
Aliases | SK 1 antibody|SK1 antibody|Sphingosine Kinase 1 antibody|SPHK 1 antibody|SPHK antibody|Sphk1 antibody|SPHK1_HUMAN antibody|SPK 1 antibody|SPK antibody|SPK1 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human Rat | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human Mouse | AssaySolutio's IHC/ICC Detection kit |
| Immunocytochemistry | 0.5-1μg/ml | Human Mouse | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P) and ICC. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-SPHK1 antibody, ASA-B1774, Western blotting
Lane 1: RAJI Cell Lysate
Lane 2: MCF-7 Cell Lysate
Lane 3: Hut Cell Lysate
Lane 4: HELA Cell Lysate
Lane 5: 293T Cell Lysate
Lane 1: RAJI Cell Lysate
Lane 2: MCF-7 Cell Lysate
Lane 3: Hut Cell Lysate
Lane 4: HELA Cell Lysate
Lane 5: 293T Cell Lysate
Anti-SPHK1 antibody, ASA-B1774, IHC(P)
IHC(P): Human Placenta Tissue
IHC(P): Human Placenta Tissue
Anti-SPHK1 antibody, ASA-B1774, ICC
ICC: HELA Cell
ICC: HELA Cell