SPARCL1 Antibody

Overview

Long Name	Antibody Type	Antibody Isotype	Host	Species Reactivity	Validated Applications	Purification
SPARC-like 1(hevin)	Polyclonal	IgG	Rabbit	Human, Mouse, Rat	IHC-P, WB	Immunogen affinity purified.
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human SPARCL1(649-664aa HCFGIKEEDIDENLLF), identical to the related mouse and rat sequences.

Properties

Form	Lyophilized
Size	100 µg/vial
Contents	Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request.
Concentration	Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration).
Storage	At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing.

Additional Information Regarding the Antigen

Gene	SPARCL1
Protein	SPARC-like protein 1
Uniprot ID	Q14515
Function
Tissue Specificity	Highly expressed in lymph node, brain, heart, lung, skeletal muscle, ovary, small intestine, and colon, with lower levels in placenta, pancreas, testis, spleen, and thymus, and no expression in kidney, liver, and peripheral blood leukocytes.
Sub-cellular localization	Secreted, extracellular space, extracellular matrix .
Sequence Similarities	Belongs to the SPARC family.
Aliases	Ecm2 antibody|Extracellular matrix protein 2 antibody|Hevin antibody|High endothelial venule protein antibody|MAST 9 antibody|mast9 antibody|Matrix glycoprotein Sc1 antibody|PIG33 antibody|proliferation inducing protein 33 antibody|RAGS1 antigen antibody|SC1 antibody|SPARC like 1 antibody|SPARC-like protein 1 antibody|Sparcl1 antibody|SPRL1_HUMAN antibody

Application Details

Application	Concentration*	Species	Validated Using**
Western blot	0.1-0.5μg/ml	Human, Mouse, Rat	AssaySolutio's ECL kit
Immunohistochemistry(Paraffin-embedded Section)	0.5-1μg/ml	Human, Rat Mouse	AssaySolutio's IHC/ICC Detection kit

AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information