More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| SPARC-like 1(hevin) | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | IHC-P, WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of human SPARCL1(649-664aa HCFGIKEEDIDENLLF), identical to the related mouse and rat sequences. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | SPARCL1 |
Protein | SPARC-like protein 1 |
Uniprot ID | Q14515 |
Function | |
Tissue Specificity | Highly expressed in lymph node, brain, heart, lung, skeletal muscle, ovary, small intestine, and colon, with lower levels in placenta, pancreas, testis, spleen, and thymus, and no expression in kidney, liver, and peripheral blood leukocytes. |
Sub-cellular localization | Secreted, extracellular space, extracellular matrix . |
Sequence Similarities | Belongs to the SPARC family. |
Aliases | Ecm2 antibody|Extracellular matrix protein 2 antibody|Hevin antibody|High endothelial venule protein antibody|MAST 9 antibody|mast9 antibody|Matrix glycoprotein Sc1 antibody|PIG33 antibody|proliferation inducing protein 33 antibody|RAGS1 antigen antibody|SC1 antibody|SPARC like 1 antibody|SPARC-like protein 1 antibody|Sparcl1 antibody|SPRL1_HUMAN antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Mouse, Rat | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human, Rat Mouse | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti-SPARCL1 antibody, ASA-B1772, IHC(P)
IHC(P): Rat Brain Tissue
IHC(P): Rat Brain Tissue
Anti-SPARCL1 antibody, ASA-B1772, Western blotting
Lane 1: Rat Lung Tissue Lysate
Lane 2: Mouse Lung Tissue Lysate
Lane 3: PANC Cell Lysate
Lane 4: A549 Cell Lysate
Lane 5: COLO320 Cell Lysate
Lane 6: MCF-7 Cell Lysate
Lane 7: HT1080 Cell Lysate
Lane 1: Rat Lung Tissue Lysate
Lane 2: Mouse Lung Tissue Lysate
Lane 3: PANC Cell Lysate
Lane 4: A549 Cell Lysate
Lane 5: COLO320 Cell Lysate
Lane 6: MCF-7 Cell Lysate
Lane 7: HT1080 Cell Lysate
Anti-SPARCL1 antibody, ASA-B1772, IHC(P)
IHC(P): Human Intestinal Cancer Tissue
IHC(P): Human Intestinal Cancer Tissue