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Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| Sp3 transcription factor | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | IHC-P, WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of human SP3 (569-599aa NTNDLTHLRVQVVDEEGDQQHQEGKRLRRVA), identical to the related mouse sequence. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | SP3 |
Protein | Transcription factor Sp3 |
Uniprot ID | Q02447 |
Function | Transcriptional factor that can act as an activator or repressor depending on isoform and/or post-translational modifications. Binds to GT and GC boxes promoter elements. Competes with SP1 for the GC-box promoters. Weak activator of transcription but can activate a number of genes involved in different processes such as cell-cycle regulation, hormone- induction and house-keeping. |
Tissue Specificity | Ubiquitously expressed. |
Sub-cellular localization | Nucleus. Nucleus, PML body. Note: Localizes to the nuclear periphery and in nuclear dots when sumoylated. Some localization in PML nuclear bodies. |
Sequence Similarities | Belongs to the Sp1 C2H2-type zinc-finger protein family. |
Aliases | D130027J01Rik antibody|DKFZp686O1631 antibody|GC binding transcription factor Sp 3 antibody|GC binding transcription factor Sp3 antibody|MGC105187 antibody|OTTMUSP00000014207 antibody|SP 3 antibody|Sp 3 transcription factor antibody|SP3 antibody|Sp3 transcription factor antibody|SP3_HUMAN antibody| Specificity protein 3 antibody|SPR 2 antibody|SPR-2 antibody|SPR2 antibody| Transcription factor SP 3 antibody|Transcription factor Sp3 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Mouse, Rat | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human, Mouse, Rat | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti- SP3 antibody, ASA-B1767, Western blotting
All lanes: Anti SP3 (ASA-B1767) at 0.5ug/ml
Lane 1: PC-12 Whole Cell Lysate at 40ug
Lane 2: HEPA Whole Cell Lysate at 40ug
Lane 3: HELA Whole Cell Lysate at 40ug
Predicted bind size: 82KD
Observed bind size: 82KD
All lanes: Anti SP3 (ASA-B1767) at 0.5ug/ml
Lane 1: PC-12 Whole Cell Lysate at 40ug
Lane 2: HEPA Whole Cell Lysate at 40ug
Lane 3: HELA Whole Cell Lysate at 40ug
Predicted bind size: 82KD
Observed bind size: 82KD
Anti- SP3 antibody, ASA-B1767, IHC(P)
IHC(P): Mouse Lung Tissue
IHC(P): Mouse Lung Tissue
Anti- SP3 antibody, ASA-B1767, IHC(P)
IHC(P): Rat Lung Tissue
IHC(P): Rat Lung Tissue