SP3  Antibody View larger

SP3 Antibody

ASA-B1767

$365.00

More info

Overview

Long Name

Antibody Type

Antibody Isotype

Host

Species Reactivity

Validated Applications

Purification 

Sp3 transcription factor Polyclonal IgG Rabbit Human, Mouse, Rat IHC-P, WB Immunogen affinity purified.

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human SP3 (569-599aa NTNDLTHLRVQVVDEEGDQQHQEGKRLRRVA), identical to the related mouse sequence.

Properties

Form

Lyophilized

Size

100 µg/vial

Contents

Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request.

Concentration

Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration).

Storage

At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing.

Additional Information Regarding the Antigen

Gene

SP3

Protein

Transcription factor Sp3

Uniprot ID

Q02447

Function

Transcriptional factor that can act as an activator or repressor depending on isoform and/or post-translational modifications. Binds to GT and GC boxes promoter elements. Competes with SP1 for the GC-box promoters. Weak activator of transcription but can activate a number of genes involved in different processes such as cell-cycle regulation, hormone- induction and house-keeping.

Tissue Specificity

Ubiquitously expressed.

Sub-cellular localization

Nucleus. Nucleus, PML body. Note: Localizes to the nuclear periphery and in nuclear dots when sumoylated. Some localization in PML nuclear bodies.

Sequence Similarities

Belongs to the Sp1 C2H2-type zinc-finger protein family.

Aliases

D130027J01Rik antibody|DKFZp686O1631 antibody|GC binding transcription factor Sp 3 antibody|GC binding transcription factor Sp3 antibody|MGC105187 antibody|OTTMUSP00000014207 antibody|SP 3 antibody|Sp 3 transcription factor antibody|SP3 antibody|Sp3 transcription factor antibody|SP3_HUMAN antibody| Specificity protein 3 antibody|SPR 2 antibody|SPR-2 antibody|SPR2 antibody| Transcription factor SP 3 antibody|Transcription factor Sp3 antibody

Application Details

Application Concentration* Species Validated Using**
Western blot 0.1-0.5μg/ml Human, Mouse, RatAssaySolutio's ECL kit
Immunohistochemistry(Paraffin-embedded Section) 0.5-1μg/ml Human, Mouse, RatAssaySolutio's IHC/ICC Detection kit

AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti- SP3 antibody, ASA-B1767, Western blotting
All lanes: Anti SP3 (ASA-B1767) at 0.5ug/ml
Lane 1: PC-12 Whole Cell Lysate at 40ug
Lane 2: HEPA Whole Cell Lysate at 40ug
Lane 3: HELA Whole Cell Lysate at 40ug
Predicted bind size: 82KD
Observed bind size: 82KD
Anti- SP3 antibody, ASA-B1767, IHC(P)
IHC(P): Mouse Lung Tissue
Anti- SP3 antibody, ASA-B1767, IHC(P)
IHC(P): Rat Lung Tissue

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