More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| sonic hedgehog | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | IHC-P, WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the N-terminus of human Sonic Hedgehog(203-218aa ATVHLEQGGTKLVKDL), identical to the related rat and mouse sequences. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | SHH |
Protein | Sonic hedgehog protein |
Uniprot ID | Q15465 |
Function | Intercellular signal essential for a variety of patterning events during development: signal produced by the notochord that induces ventral cell fate in the neural tube and somites, and the polarizing signal for patterning of the anterior- posterior axis of the developing limb bud. Displays both floor plate- and motor neuron-inducing activity. The threshold concentration of N-product required for motor neuron induction is 5-fold lower than that required for floor plate induction. Activates the transcription of target genes by interacting with its receptor PTCH1 to prevent normal inhibition by PTCH1 on the constitutive signaling activity of SMO (By similarity). |
Tissue Specificity | Expressed in fetal intestine, liver, lung, and kidney. Not expressed in adult tissues. |
Sub-cellular localization | Sonic hedgehog protein C-product: Secreted, extracellular space . Note: The C-terminal peptide diffuses from the cell. |
Sequence Similarities | Belongs to the hedgehog family. |
Aliases | HHG 1 antibody|HHG-1 antibody|HHG1 antibody|HLP 3 antibody|HLP3 antibody|Holoprosencephaly 3 antibody|HPE 3 antibody|HPE3 antibody|MCOPCB5 antibody|SHH antibody|SHH_HUMAN antibody|SMMC I antibody|SMMCI antibody|Sonic Hedgehog(Drosophila) homolog antibody|sonic hedgehog homolog(Drosophila) antibody|Sonic hedgehog homolog antibody|Sonic hedgehog protein antibody|Sonic hedgehog protein C-product antibody|TPT antibody|TPTPS antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Rat Mouse | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human, Rat Mouse | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti-Sonic Hedgehog antibody, ASA-B1757, Western blotting
Lane 1: Rat Liver Tissue Lysate
Lane 2: Rat Intestine Tissue Lysate
Lane 3: HELA Cell Lysate
Lane 4: A549 Cell Lysate
Lane 5: SMMC Cell Lysate
Lane 6: MM231 Cell Lysate
Lane 1: Rat Liver Tissue Lysate
Lane 2: Rat Intestine Tissue Lysate
Lane 3: HELA Cell Lysate
Lane 4: A549 Cell Lysate
Lane 5: SMMC Cell Lysate
Lane 6: MM231 Cell Lysate
Anti-Sonic Hedgehog antibody, ASA-B1757, IHC(P)
IHC(P): Rat Brain Tissue
IHC(P): Rat Brain Tissue
Anti-Sonic Hedgehog antibody, ASA-B1757, IHC(P)
IHC(P): Human Mammary Tissue
IHC(P): Human Mammary Tissue