SOCS3 Antibody View larger

SOCS3 Antibody

ASA-B1747

$330.00

More info

Overview

Long Name

Antibody Type

Antibody Isotype

Host

Species Reactivity

Validated Applications

Purification 

suppressor of cytokine signaling 3 Polyclonal IgG Rabbit Human, Mouse IHC-P, WB Immunogen affinity purified.

Immunogen

A synthetic peptide corresponding to a sequence in the middle region of human SOCS3(71-85aa RDSSDQRHFFTLSVK), identical to the related mouse sequence, different from the related rat sequence by one amino acid.

Properties

Form

Lyophilized

Size

100 µg/vial

Contents

Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request.

Concentration

Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration).

Storage

At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing.

Additional Information Regarding the Antigen

Gene

SOCS3

Protein

Suppressor of cytokine signaling 3(SOCS-3)

Uniprot ID

O14543

Function

SOCS family proteins form part of a classical negative feedback system that regulates cytokine signal transduction. SOCS3 is involved in negative regulation of cytokines that signal through the JAK/STAT pathway. Inhibits cytokine signal transduction by binding to tyrosine kinase receptors including gp130, LIF, erythropoietin, insulin, IL12, GCSF and leptin receptors. Binding to JAK2 inhibits its kinase activity. Suppresses fetal liver erythropoiesis. Regulates onset and maintenance of allergic responses mediated by T-helper type 2 cells. Regulates IL-6 signaling in vivo (By similarity). Probable substrate recognition component of a SCF-like ECS (Elongin BC- CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Seems to recognize IL6ST (By similarity).

Tissue Specificity

Widely expressed with high expression in heart, placenta, skeletal muscle, peripheral blood leukocytes, fetal and adult lung, and fetal liver and kidney. Lower levels in thymus.

Sub-cellular localization

Sequence Similarities

Contains 1 SH2 domain.

Aliases

ATOD4 antibody|CIS 3 antibody|CIS-3 antibody|CIS3 antibody|Cish3 antibody|Cytokine induced SH2 protein 3 antibody|Cytokine-inducible SH2 protein 3 antibody|E2a Pbx1 target gene in fibroblasts 10 antibody|EF 10 antibody|MGC71791 antibody|SOCS 3 antibody|SOCS-3 antibody|Socs3 antibody|SOCS3_HUMAN antibody|SSI 3 antibody|SSI-3 antibody|SSI3 antibody|STAT induced STAT inhibitor 3 antibody|STAT-induced STAT inhibitor 3 antibody|Suppressor of cytokine signaling 3 antibody

Application Details

Application Concentration* Species Validated Using**
Western blot 0.1-0.5μg/ml Human MouseAssaySolutio's ECL kit
Immunohistochemistry(Paraffin-embedded Section) 0.5-1μg/ml Human MouseAssaySolutio's IHC/ICC Detection kit

AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti-SOCS3 antibody, ASA-B1747, Western blotting
Lane 1: JURKAT Cell Lysate
Lane 2: CEM Cell Lysate
Lane 3: RAJI Cell Lysate
Anti-SOCS3 antibody, ASA-B1747, IHC(P)
IHC(P): Human Tonsil Tissue

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