More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily b, member 1 | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | IHC-P, WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of human SNF5(362-377aa EKKIRDQDRNTRRMRR), identical to the related rat and mouse sequences. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | SMARCB1 |
Protein | SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily B member 1 |
Uniprot ID | Q12824 |
Function | Core component of the BAF (hSWI/SNF) complex. This ATP- dependent chromatin-remodeling complex plays important roles in cell proliferation and differentiation, in cellular antiviral activities and inhibition of tumor formation. The BAF complex is able to create a stable, altered form of chromatin that constrains fewer negative supercoils than normal. This change in supercoiling would be due to the conversion of up to one-half of the nucleosomes on polynucleosomal arrays into asymmetric structures, termed altosomes, each composed of 2 histones octamers. Stimulates in vitro the remodeling activity of SMARCA4/BRG1/BAF190A. Involved in activation of CSF1 promoter. Belongs to the neural progenitors- specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a postmitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to postmitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth (By similarity). Plays a key role in cell-cycle control and causes cell cycle arrest in G0/G1. |
Tissue Specificity | |
Sub-cellular localization | Nucleus. |
Sequence Similarities | Belongs to the SNF5 family. |
Aliases | BAF47 antibody|BRG1-associated factor 47 antibody|hSNF5 antibody|INI1 antibody|Integrase interactor 1 protein antibody|Malignant rhabdoid tumor suppressor antibody|RDT antibody|RTPS1 antibody|Sfh1p antibody|SMARCB1 antibody|SNF5 homolog antibody|SNF5_HUMAN antibody|SNF5L1 antibody|Snr1 antibody|Sucrose nonfermenting yeast homolog like 1 antibody|SWI/SNF complex component SNF5 antibody|SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily B member 1 antibody|SWI10 antibody|Transcription factor TYE4 antibody|Transcription regulatory protein SNF5 antibody|TYE4 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Rat Mouse | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human, Rat Mouse | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti-SNF5 antibody, ASA-B1741, Western blotting
Recombinant Protein Detection Source: E.coli derived -recombinant human SMARCB1, 41.0KD(162aa tag+ L186-W385)
Lane 1: Recombinant Human SMARCB1 Protein 10ng
Lane 2: Recombinant Human SMARCB1 Protein 5ng
Lane 3: Recombinant Human SMARCB1 Protein 2.5ng
Recombinant Protein Detection Source: E.coli derived -recombinant human SMARCB1, 41.0KD(162aa tag+ L186-W385)
Lane 1: Recombinant Human SMARCB1 Protein 10ng
Lane 2: Recombinant Human SMARCB1 Protein 5ng
Lane 3: Recombinant Human SMARCB1 Protein 2.5ng
Anti-SNF5 antibody, ASA-B1741, Western blotting
Lane 1: Rat Kidney Tissue Lysate
Lane 2: Human Placenta Tissue Lysate
Lane 3: Rat Spleen Tissue Lysate
Lane 4: PC-12 Cell Lysate
Lane 5: HELA Cell Lysate
Lane 6: JURKAT Cell Lysate
Lane 7: 293T Cell Lysate
Lane 1: Rat Kidney Tissue Lysate
Lane 2: Human Placenta Tissue Lysate
Lane 3: Rat Spleen Tissue Lysate
Lane 4: PC-12 Cell Lysate
Lane 5: HELA Cell Lysate
Lane 6: JURKAT Cell Lysate
Lane 7: 293T Cell Lysate
Anti-SNF5 antibody, ASA-B1741, IHC(P)
IHC(P): Rat Brain Tissue
IHC(P): Rat Brain Tissue