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Smad2 Antibody

ASA-B1728

$330.00

More info

Overview

Long Name

Antibody Type

Antibody Isotype

Host

Species Reactivity

Validated Applications

Purification 
SMAD family member 2 Polyclonal IgG Rabbit Human, Mouse, Rat IHC-P, ICC, WB Immunogen affinity purified.

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminus of human Smad2(94-112aa DQWDTTGLYSFSEQTRSLD), identical to the related rat and mouse sequences.

Properties

Form

 Lyophilized

Size

 100 µg/vial

Contents

 Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request.

Concentration

 Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration).

Storage

 At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing.

Additional Information Regarding the Antigen

Gene

 SMAD2

Protein

 Mothers against decapentaplegic homolog 2

Uniprot ID

 Q15796

Function

 Receptor-regulated SMAD (R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases. Binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD2/SMAD4 complex, activates transcription. May act as a tumor suppressor in colorectal carcinoma. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator.

Tissue Specificity

Expressed at high levels in skeletal muscle, endothelial cells, heart and placenta.

Sub-cellular localization

 Cytoplasm. Nucleus. Note: Cytoplasmic and nuclear in the absence of TGF-beta. On TGF-beta stimulation, migrates to the nucleus when complexed with SMAD4. On dephosphorylation by phosphatase PPM1A, released from the SMAD2/SMAD4 complex, and exported out of the nucleus by interaction with RANBP1.

Sequence Similarities

 Belongs to the dwarfin/SMAD family.

Aliases

 hMAD 2 antibody|hMAD-2 antibody|hSMAD2 antibody|JV18 1 antibody|JV18 antibody|JV18-1 antibody|JV181 antibody|MAD antibody|MAD homolog 2 antibody|MAD Related Protein 2 antibody|Mad-related protein 2 antibody|MADH2 antibody|MADR2 antibody|MGC22139 antibody|MGC34440 antibody|Mothers Against Decapentaplegic Homolog 2 antibody|mothers against DPP homolog 2 antibody|OTTHUMP00000163489 antibody|Sma and Mad related protein 2 antibody|SMAD 2 antibody|SMAD antibody|SMAD family member 2 antibody|SMAD, mothers against DPP homolog 2 antibody|SMAD2 antibody|SMAD2_HUMAN antibody

Application Details

Application Concentration* Species Validated Using**
Western blot 0.1-0.5μg/ml Human, Rat MouseAssaySolutio's ECL kit
Immunohistochemistry(Paraffin-embedded Section) 0.5-1μg/ml Human Mouse, RatAssaySolutio's IHC/ICC Detection kit
Immunocytochemistry 0.5-1μg/ml Human Mouse, RatAssaySolutio's IHC/ICC Detection kit

AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P) and ICC. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-Smad2 antibody, ASA-B1728, Western blotting
Recombinant Protein Detection Source: E.coli derived -recombinant human SHC1, 35.0KD(162aa tag+D424-P578)
Lane 1: Recombinant Human SHC1 Proteins 10ng
Lane 2: Recombinant Human SHC1 Proteins 5ng
Lane 3: Recombinant Human SHC1 Proteins 2.5ng
Anti-Smad2 antibody, ASA-B1728, IHC(P)
IHC(P): Human Mammary Cancer Tissue
Anti-Smad2 antibody, ASA-B1728, Western blotting
Lane 1: Rat Brain Tissue Lysate
Lane 2: HELA Cell Lysate
Lane 3: SMMC Cell Lysate
Lane 4: JURKAT Cell Lysate
Lane 5: U87 Cell Lysate
Lane 6: MCF-7 Cell Lysate

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