More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| solute carrier family 9, subfamily A(NHE2, cation proton antiporter 2), member 2 | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | IHC-P, WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of human SLC9A2(666-684aa RYLSLPKNTKLPEKLQKRR), different from the related rat and mouse sequences by two amino acids. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | SLC9A2 |
Protein | Sodium/hydrogen exchanger 2 |
Uniprot ID | Q9UBY0 |
Function | Involved in pH regulation to eliminate acids generated by active metabolism or to counter adverse environmental conditions. Major proton extruding system driven by the inward sodium ion chemical gradient. Seems to play an important role in colonic sodium absorption. |
Tissue Specificity | Expressed in skeletal muscle, colon and kidney. Lower levels in the testis, prostate, ovary, and small intestine. |
Sub-cellular localization | Membrane; Multi-pass membrane protein. |
Sequence Similarities | Belongs to the monovalent cation:proton antiporter 1 (CPA1) transporter (TC 2.A.36) family. |
Aliases | H7 antibody|Na(+)/H(+) exchanger 2 antibody|NHE 2 antibody|NHE-2 antibody|NHE2 antibody|SL9A2_HUMAN antibody|Slc9a2 antibody|Sodium/hydrogen exchanger antibody|Sodium/hydrogen exchanger 2 antibody|Solute carrier family 9(sodium/hydrogen exchanger), member 2 antibody|Solute carrier family 9 member 2 antibody|Solute carrier family 9, member 2 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Rat Human, Mouse | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human, Rat Mouse | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti-SLC9A2 antibody, ASA-B1721, Western blotting
Lane 1: Rat Skeletal Musle Tissue Lysate
Lane 2: Rat Kidney Tissue Lysate
Lane 1: Rat Skeletal Musle Tissue Lysate
Lane 2: Rat Kidney Tissue Lysate
Anti-SLC9A2 antibody, ASA-B1721, IHC(P)
IHC(P): Human Lung Cancer Tissue
IHC(P): Human Lung Cancer Tissue
