More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| solute carrier family 9, subfamily A (NHE1, cation proton antiporter 1), member 1 | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | IHC-P, WB | Immunogen affinity purified. |
Immunogen | ||||||
| E.coli-derived human SLC9A1 recombinant protein (Position: H543-Q815). Human SLC9A1 shares 92% and 93% amino acid (aa) sequences identity with mouse and rat SLC9A1, respectively. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | SLC9A1 |
Protein | Sodium/hydrogen exchanger 1 |
Uniprot ID | P19634 |
Function | Involved in pH regulation to eliminate acids generated by active metabolism or to counter adverse environmental conditions. Major proton extruding system driven by the inward sodium ion chemical gradient. Plays an important role in signal transduction. |
Tissue Specificity | Kidney and intestine. |
Sub-cellular localization | Membrane; Multi-pass membrane protein. Endoplasmic reticulum membrane ; Multi-pass membrane protein . Cell membrane; Multi-pass membrane protein. Note: Colocalizes with CHP1 at the reticulum endoplasmic (By similarity). Colocalizes with CHP1 and CHP2 at the plasma membrane. |
Sequence Similarities | Belongs to the monovalent cation:proton antiporter 1 (CPA1) transporter (TC 2.A.36) family. |
Aliases | amiloride-sensitive antibody|APNH antibody|APNH1 antibody|FLJ42224 antibody|Na Li countertransporter antibody|Na(+)/H(+) antiporter antibody|Na(+)/H(+) exchanger 1 antibody|Na+ H+ antiporter amiloride-sensitive antibody|Na+ H+ antiporter antibody|Na+ H+ exchanger 1 antibody|NHE-1 antibody|NHE1 antibody|OTTHUMP00000004468 antibody|SL9A1_HUMAN antibody|SLC9A1 antibody|Sodium hydrogen exchanger 1 antibody|Sodium/hydrogen exchanger 1 antibody|solute carrier family 9 antibody|Solute carrier family 9 member 1 antibody|Solute carrier family 9 sodium hydrogen exchanger isoform 1 antiporter Na+ H+ amiloride sensitive antibody|Solute carrier family 9 subfamily A (NHE1 cation proton antiporter 1) member 1 antibody|Solute carrier family 9 subfamily A member 1 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Rat | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human, Mouse, Rat | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti-SLC9A1 antibody, ASA-B1720--1.jpg
All lanes: Anti SLC9A1 (ASA-B1720) at 0.5ug/ml
WB: Recombinant Human Sodium Protein 0.5ng
Predicted bind size: 37KD
Observed bind size: 37KD
All lanes: Anti SLC9A1 (ASA-B1720) at 0.5ug/ml
WB: Recombinant Human Sodium Protein 0.5ng
Predicted bind size: 37KD
Observed bind size: 37KD
Anti-SLC9A1 antibody, ASA-B1720--2.jpg
All lanes: Anti SLC9A1 (ASA-B1720) at 0.5ug/ml
Lane 1: PC-12 Whole Cell Lysate at 40ug
Lane 2: MCF-7 Whole Cell Lysate at 40ug
Predicted bind size: 91KD
Observed bind size: 91KD
All lanes: Anti SLC9A1 (ASA-B1720) at 0.5ug/ml
Lane 1: PC-12 Whole Cell Lysate at 40ug
Lane 2: MCF-7 Whole Cell Lysate at 40ug
Predicted bind size: 91KD
Observed bind size: 91KD
Anti-SLC9A1 antibody, ASA-B1720--3.jpg
IHC(P): Rat Kidney Tissue
IHC(P): Rat Kidney Tissue