SLC6A4  Antibody View larger

SLC6A4 Antibody

ASA-B1718

$365.00

More info

Overview

Long Name

Antibody Type

Antibody Isotype

Host

Species Reactivity

Validated Applications

Purification 

solute carrier family 6 (neurotransmitter transporter, serotonin), member 4 Polyclonal IgG Rabbit Human, Rat IHC-P, WB Immunogen affinity purified.

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminus of human SLC6A4 (57-85aa DDTRHSIPATTTTLVAELHQGERETWGKK), different from the related mouse and rat sequences by six amino acids.

Properties

Form

Lyophilized

Size

100 µg/vial

Contents

Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request.

Concentration

Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration).

Storage

At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing.

Additional Information Regarding the Antigen

Gene

SLC6A4

Protein

Sodium-dependent serotonin transporter

Uniprot ID

P31645

Function

Serotonin transporter whose primary function in the central nervous system involves the regulation of serotonergic signaling via transport of serotonin molecules from the synaptic cleft back into the pre-synaptic terminal for re-utilization. Plays a key role in mediating regulation of the availability of serotonin to other receptors of serotonergic systems. Terminates the action of serotonin and recycles it in a sodium-dependent manner.

Tissue Specificity

Expressed in platelets (at protein level).

Sub-cellular localization

Cell membrane; Multi-pass membrane protein. Endomembrane system; Multi-pass membrane protein. Endosome membrane; Multi-pass membrane protein. Note: Translocates from intracellular locations to the plasma membrane. Density of transporter molecules on the plasma membrane is itself regulated by serotonin.

Sequence Similarities

Belongs to the sodium:neurotransmitter symporter (SNF) (TC 2.A.22) family. SLC6A4 subfamily.

Aliases

5 HTT antibody|5 HTTLPR antibody|5 hydroxytryptamine (serotonin) transporter antibody|5 hydroxytryptamine transporter antibody|5HT transporter antibody|5HTT antibody|hSERT antibody|HTT antibody|Na+/Cl- dependent serotonin transporter antibody|OCD1 antibody| SC6A4_HUMAN antibody|Serotonin transporter 1 antibody|SERT antibody|SERT1 antibody|SLC6A4 antibody|Sodium dependent serotonin transporter antibody|Sodium-dependent serotonin transporter antibody| Solute carrier family 6 (neurotransmitter transporter) member 4 antibody|solute carrier family 6 (neurotransmitter transporter, serotonin), member 4 antibody| Solute carrier family 6 member 4 antibody

Application Details

Application Concentration* Species Validated Using**
Western blot 0.1-0.5μg/ml HumanAssaySolutio's ECL kit
Immunohistochemistry(Paraffin-embedded Section) 0.5-1μg/ml Human, RatAssaySolutio's IHC/ICC Detection kit

AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti- SLC6A4 antibody, ASA-B1718, Western blotting
All lanes: Anti SLC6A4 (ASA-B1718) at 0.5ug/ml
Lane 1: A549 Whole Cell Lysate at 40ug
Lane 2: MM231 Whole Cell Lysate at 40ug
Lane 3: HELA Whole Cell Lysate at 40ug
Lane 4: 22RV1 Whole Cell Lysate at 40ug
Predicted bind size: 70KD
Observed bind size: 95KD
Anti- SLC6A4 antibody, ASA-B1718, IHC(P)
IHC(P): Rat Brain Tissue
Anti- SLC6A4 antibody, ASA-B1718, IHC(P)
IHC(P): Human Mammary Cancer Tissue

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