More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| solute carrier family 16, member 4(monocarboxylic acid transporter 5) | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | IHC-P, WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the N-terminus of human SLC16A4(134-148aa VVTTKYFKKRLALST), different from the related rat and mouse sequences by two amino acids. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | SLC16A4 |
Protein | Monocarboxylate transporter 5 |
Uniprot ID | O15374 |
Function | Proton-linked monocarboxylate transporter. Catalyzes the rapid transport across the plasma membrane of many monocarboxylates such as lactate, pyruvate, branched-chain oxo acids derived from leucine, valine and isoleucine, and the ketone bodies acetoacetate, beta-hydroxybutyrate and acetate (By similarity). |
Tissue Specificity | |
Sub-cellular localization | Cell membrane; Multi-pass membrane protein. |
Sequence Similarities | |
Aliases | MCT4 antibody|MCT5 antibody|Monocarboxylate transporter 4|Monocarboxylate transporter 5|SLC16A4|Solute carrier family 16 member 4|solute carrier family 16, member 4|SOLUTE CARRIER FAMILY 16(MONOCARBOXYLIC ACID TRANSPORTER), MEMBER 4 |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Rat Mouse | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human, Rat Mouse | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti-SLC16A4 antibody, ASA-B1707, Western blotting
Lane 1: Rat Testis Tissue Lysate
Lane 2: JURKAT Cell Lysate
Lane 3: HELA Cell Lysate
Lane 4: MCF-7 Cell Lysate
Lane 1: Rat Testis Tissue Lysate
Lane 2: JURKAT Cell Lysate
Lane 3: HELA Cell Lysate
Lane 4: MCF-7 Cell Lysate
Anti-SLC16A4 antibody, ASA-B1707, IHC(P)
IHC(P): Human Mammary Cancer Tissue
IHC(P): Human Mammary Cancer Tissue
Anti-SLC16A4 antibody, ASA-B1707, IHC(P)
IHC(P): Human Placenta Cancer Tissue
IHC(P): Human Placenta Cancer Tissue