SLC16A3  Antibody View larger

SLC16A3 Antibody

ASA-B1706

$365.00

More info

Overview

Long Name

Antibody Type

Antibody Isotype

Host

Species Reactivity

Validated Applications

Purification 

solute carrier family 16 (monocarboxylate transporter), member 3 Polyclonal IgG Rabbit Human, Mouse, Rat IHC-P, WB Immunogen affinity purified.

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminal of human SLC16A3(438-465aa VDLREVEHFLKAEPEKNGEVVHTPETSV), different from the related mouse and rat sequences by one amino acid.

Properties

Form

Lyophilized

Size

100 µg/vial

Contents

Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request.

Concentration

Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration).

Storage

At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing.

Additional Information Regarding the Antigen

Gene

SLC1A1

Protein

Monocarboxylate transporter 4

Uniprot ID

O15427

Function

Proton-linked monocarboxylate transporter. Catalyzes the rapid transport across the plasma membrane of many monocarboxylates such as lactate, pyruvate, branched-chain oxo acids derived from leucine, valine and isoleucine, and the ketone bodies acetoacetate, beta-hydroxybutyrate and acetate (By similarity).

Tissue Specificity

Highly expressed in skeletal muscle.

Sub-cellular localization

Cell membrane; Multi-pass membrane protein.

Sequence Similarities

Belongs to the major facilitator superfamily. Monocarboxylate porter (TC 2.A.1.13) family.

Aliases

MCT 4 antibody|MCT3 antibody|MCT4 antibody|Monocarboxylate transporter 3 antibody|Monocarboxylate transporter 4 antibody|MOT4_HUMAN antibody|SLC16A3 antibody|Solute carrier family 16 member 3 antibody

Application Details

Application Concentration* Species Validated Using**
Western blot 0.1-0.5μg/ml Human, Mouse, RatAssaySolutio's ECL kit
Immunohistochemistry(Paraffin-embedded Section) 0.5-1μg/ml HumanAssaySolutio's IHC/ICC Detection kit

AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti- SLC16A3 antibody, ASA-B1706, Western blotting
All lanes: Anti SLC16A3 (ASA-B1706) at 0.5ug/ml
WB: Recombinant Human SLC16A3 Protein 0.5ng
Predicted bind size: 45KD
Observed bind size: 45KD
Anti- SLC16A3 antibody, ASA-B1706, Western blotting
All lanes: Anti SLC16A3 (ASA-B1706) at 0.5ug/ml
Lane 1: HELA Whole Cell Lysate at 40ug
Lane 2: JURKAT Whole Cell Lysate at 40ug
Lane 3: K562 Whole Cell Lysate at 40ug
Lane 4: MM231 Whole Cell Lysate at 40ug
Lane 5: HL-60 Whole Cell Lysate at 40ug
Lane 6: Rat Brain Tissue Lysate at 50ug
Lane 7: Mouse Brain Tissue Lysate at 50ug
Predicted bind size: 49KD
Observed bind size: 49KD
Anti- SLC16A3 antibody, ASA-B1706, IHC(P)
IHC(P): Human Lung Cancer Tissue

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