More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| solute carrier family 12(sodium/chloride transporters), member 3 | Polyclonal | IgG | Rabbit | Human, Rat | IHC-P, IHC-F, WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the N-terminus of human SLC12A3(310-328aa EDKASKGFFSYRADIFVQN), different from the related mouse sequence by three amino acids, and from the related rat sequence by four amino acids. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | SLC12A3 |
Protein | Solute carrier family 12 member 3 |
Uniprot ID | P55017 |
Function | Key mediator of sodium and chloride reabsorption in this nephron segment, accounting for a significant fraction of renal sodium reabsorption. |
Tissue Specificity | Predominant in kidney. |
Sub-cellular localization | Cell membrane; Multi-pass membrane protein. |
Sequence Similarities | |
Aliases | FLJ96318 antibody|Na Cl symporter antibody|Na-Cl symporter antibody|NaCl electroneutral thiazide sensitive cotransporter antibody|NCCT antibody|S12A3_HUMAN antibody|slc12a3 antibody|Solute carrier family 12(sodium/chloride transporters) member 3 antibody|Solute carrier family 12 member 3 antibody|Thiazide sensitive Na Cl cotransporter antibody|Thiazide sensitive sodium chloride cotransporter antibody|Thiazide-sensitive sodium-chloride cotransporter antibody|TSC antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Rat | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human, Rat | AssaySolutio's IHC/ICC Detection kit |
| Immunohistochemistry(Frozen Section) | 0.5-1μg/ml | Rat Human | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P) and IHC(F). *Blocking peptide can be purchased at $65. Contact us for more information
Anti-SLC12A3 antibody, ASA-B1703, Western blotting
Lane 1: NRK Cell Lysate
Lane 2: SMMC Cell Lysate
Lane 1: NRK Cell Lysate
Lane 2: SMMC Cell Lysate
Anti-SLC12A3 antibody, ASA-B1703, IHC(P)
IHC(P): Rat Kidney Tissue
IHC(P): Rat Kidney Tissue
Anti-SLC12A3 antibody, ASA-B1703, IHC(F)
IHC(F): Rat Kidney Tissue
IHC(F): Rat Kidney Tissue