SKP2 Antibody View larger

SKP2 Antibody

ASA-B1698

$330.00

More info

Overview

Long Name

Antibody Type

Antibody Isotype

Host

Species Reactivity

Validated Applications

Purification 

S-phase kinase-associated protein 2, E3 ubiquitin protein ligase Polyclonal IgG Rabbit Human, Mouse, Rat IHC-P, WB Immunogen affinity purified.

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminus of human SKP2(2-20aa HRKHLQEIPDLSSNVATSF), different from the related rat and mouse sequences by three amino acids.

Properties

Form

Lyophilized

Size

100 µg/vial

Contents

Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request.

Concentration

Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration).

Storage

At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing.

Additional Information Regarding the Antigen

Gene

SKP2

Protein

S-phase kinase-associated protein 2

Uniprot ID

Q13309

Function

Substrate recognition component of a SCF (SKP1-CUL1-F- box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins involved in cell cycle progression, signal transduction and transcription. Specifically recognizes phosphorylated CDKN1B/p27kip and is involved in regulation of G1/S transition. Degradation of CDKN1B/p27kip also requires CKS1. Recognizes target proteins ORC1, CDT1, RBL2, KMT2A/MLL1, CDK9, RAG2, FOXO1, UBP43, and probably MYC, TOB1 and TAL1. Degradation of TAL1 also requires STUB1. Recognizes CDKN1A in association with CCNE1 or CCNE2 and CDK2. Promotes ubiquitination and destruction of CDH1 in a CK1-Dependent Manner, thereby regulating cell migration.

Tissue Specificity

Sub-cellular localization

Cytoplasm.

Sequence Similarities

Contains 1 F-box domain.

Aliases

CDK2/Cyclin A associated protein p45 antibody|Cyclin A/CDK2 associated protein p45 antibody|Cyclin-A/CDK2-associated protein p45 antibody|F box protein Skp2 antibody|F box/LRR repeat protein 1 antibody|F-box protein Skp2 antibody|F-box/LRR-repeat protein 1 antibody|FBL 1 antibody|FBL1 antibody|FBXL 1 antibody|FBXL1 antibody|FLB 1 antibody|FLB1 antibody|MGC1366 antibody|p45 antibody|p45skp2 antibody|S phase kinase associated protein 2 antibody|S-phase kinase-associated protein 2 antibody|SKP 2 antibody|Skp2 antibody|SKP2_HUMAN antibody

Application Details

Application Concentration* Species Validated Using**
Western blot 0.1-0.5μg/ml Human, Rat MouseAssaySolutio's ECL kit
Immunohistochemistry(Paraffin-embedded Section) 0.5-1μg/ml Human, Rat MouseAssaySolutio's IHC/ICC Detection kit

AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti-SKP2 antibody, ASA-B1698, Western blotting
WB: MCF-7 Cell Lysate
Anti-SKP2 antibody, ASA-B1698, IHC(P)
IHC(P): Human Mammary Cancer Tissue

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