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Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| sirtuin 6 | Polyclonal | IgG | Rabbit | Human, Mouse | IHC-P, WB | Immunogen affinity purified. |
Immunogen | ||||||
| E.coli-derived human SIRT6 recombinant protein (Position: D14-E180). Human SIRT6 shares 95% amino acid (aa) sequence identity with mouse SIRT6. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | SIRT6 |
Protein | NAD-dependent protein deacetylase sirtuin-6 |
Uniprot ID | Q8N6T7 |
Function | NAD-dependent protein deacetylase. Has deacetylase activity towards histone H3K9Ac and H3K56Ac. Modulates acetylation of histone H3 in telomeric chromatin during the S-phase of the cell cycle. Deacetylates histone H3K9Ac at NF-kappa-B target promoters and may down-regulate the expression of a subset of NF- kappa-B target genes. Acts as a corepressor of the transcription factor HIF1A to control the expression of multiple glycolytic genes to regulate glucose homeostasis. Required for genomic stability. Regulates the production of TNF protein. Has a role in the regulation of life span (By similarity). Deacetylation of nucleosomes interferes with RELA binding to target DNA. May be required for the association of WRN with telomeres during S-phase and for normal telomere maintenance. Required for genomic stability. Required for normal IGF1 serum levels and normal glucose homeostasis. Modulates cellular senescence and apoptosis. On DNA damage, promotes DNA end resection via deacetylation of RBBP8. Has very weak deacetylase activity and can bind NAD(+) in the absence of acetylated substrate. |
Tissue Specificity | |
Sub-cellular localization | Nucleus, nucleoplasm. |
Sequence Similarities | Belongs to the sirtuin family. Class IV subfamily. |
Aliases | 2810449N18Rik antibody|AI043036 antibody|Mono ADP ribosyltransferase sirtuin 6 antibody|Mono-ADP-ribosyltransferase sirtuin-6 antibody|NAD-dependent deacetylase sirtuin-6 antibody|NAD-dependent protein deacetylase sirtuin-6 antibody|Regulatory proteinSIR2 homolog antibody|SIR2 like 6 antibody|SIR2 like protein 6 antibody|Sir2 related protein type 6 antibody|SIR2-like protein 6 antibody|SIR2L6 antibody|SIRT 6 antibody|SIRT6 antibody|SIRT6_HUMAN antibody|Sirtuin (silent mating type information regulation 2 homolog) 6 (S. cerevisiae) antibody|Sirtuin 6 antibody|Sirtuin type 6 antibody|Sirtuin6 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human, Mouse | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti- SIRT6 antibody, ASA-B1696, Western blotting
All lanes: Anti SIRT6 (ASA-B1696) at 0.5ug/ml
Lane 1: HELA Whole Cell Lysate at 40ug
Lane 2: A549 Whole Cell Lysate at 40ug
Lane 3: Human Placenta Tissue Lysate at 50ug
Predicted bind size: 39KD
Observed bind size: 39KD
All lanes: Anti SIRT6 (ASA-B1696) at 0.5ug/ml
Lane 1: HELA Whole Cell Lysate at 40ug
Lane 2: A549 Whole Cell Lysate at 40ug
Lane 3: Human Placenta Tissue Lysate at 50ug
Predicted bind size: 39KD
Observed bind size: 39KD
Anti- SIRT6 antibody, ASA-B1696, IHC(P)
IHC(P): Mouse Cardiac Muscle Tissue
IHC(P): Mouse Cardiac Muscle Tissue
Anti- SIRT6 antibody, ASA-B1696, IHC(P)
IHC(P): Human Mammary Cancer Tissue
IHC(P): Human Mammary Cancer Tissue