More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| SHC(Src homology 2 domain containing) transforming protein 1 | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | IHC-P, IHC-F, ICC, WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of human SHC(424-440aa DPSYVNVQNLDKARQAV), different from the related rat and mouse sequences by two amino acids. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | SHC1 |
Protein | SHC-transforming protein 1 |
Uniprot ID | P29353 |
Function | Signaling adapter that couples activated growth factor receptors to signaling pathways. Participates in a signaling cascade initiated by activated KIT and KITLG/SCF. Isoform p46Shc and isoform p52Shc, once phosphorylated, couple activated receptor tyrosine kinases to Ras via the recruitment of the GRB2/SOS complex and are implicated in the cytoplasmic propagation of mitogenic signals. Isoform p46Shc and isoform p52Shc may thus function as initiators of the Ras signaling cascade in various non-neuronal systems. Isoform p66Shc does not mediate Ras activation, but is involved in signal transduction pathways that regulate the cellular response to oxidative stress and life span. Isoform p66Shc acts as a downstream target of the tumor suppressor p53 and is indispensable for the ability of stress-activated p53 to induce elevation of intracellular oxidants, cytochrome c release and apoptosis. The expression of isoform p66Shc has been correlated with life span (By similarity). Participates in signaling downstream of the angiopoietin receptor TEK/TIE2, and plays a role in the regulation of endothelial cell migration and sprouting angiogenesis. |
Tissue Specificity | Widely expressed. Expressed in neural stem cells but absent in mature neurons. |
Sub-cellular localization | Cytoplasm. |
Sequence Similarities | Contains 1 PID domain. |
Aliases | FLJ26504 antibody|MGC98812 antibody|p46 antibody|p52 antibody|p52 SHC antibody|p52SHC antibody|p66 antibody|p66 SHC antibody|p66SHC antibody|SH2 domain protein C1 antibody|SHC(Src homology 2 domain containing) transforming protein 1 antibody|SHC 1 antibody|SHC A antibody|Shc antibody|SHC transforming protein 1 antibody|SHC transforming protein antibody|SHC-transforming protein 1 antibody|SHC-transforming protein 3 antibody|SHC-transforming protein A antibody|SHC1 antibody|SHC1_HUMAN antibody|SHCA antibody|Src homology 2 domain containing transforming protein C1 antibody|Src homology 2 domain-containing-transforming protein C1 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Rat Mouse | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human, Rat Mouse | AssaySolutio's IHC/ICC Detection kit |
| Immunohistochemistry(Frozen Section) | 0.5-1μg/ml | Rat Human, Mouse | AssaySolutio's IHC/ICC Detection kit |
| Immunocytochemistry | 0.5-1μg/ml | Human Human, Mouse | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P) IHC(F) and ICC. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-SHC antibody, ASA-B1685, Western blotting
Recombinant Protein Detection Source: E.coli derived -recombinant human SHC1, 35.0KD(162aa tag+D424-P578)
Lane 1: Recombinant Human SHC1 Proteins 10ng
Lane 2: Recombinant Human SHC1 Proteins 5ng
Lane 3: Recombinant Human SHC1 Proteins 2.5ng
Recombinant Protein Detection Source: E.coli derived -recombinant human SHC1, 35.0KD(162aa tag+D424-P578)
Lane 1: Recombinant Human SHC1 Proteins 10ng
Lane 2: Recombinant Human SHC1 Proteins 5ng
Lane 3: Recombinant Human SHC1 Proteins 2.5ng
Anti-SHC antibody, ASA-B1685, Western blotting
Lane 1: Rat Brain Tissue Lysate
Lane 2: A549 Cell Lysate
Lane 3: A431 Cell Lysate
Lane 4: 293T Cell Lysate
Lane 5: HELA Cell Lysate
Lane 6: JURKAT Cell Lysate
Lane 1: Rat Brain Tissue Lysate
Lane 2: A549 Cell Lysate
Lane 3: A431 Cell Lysate
Lane 4: 293T Cell Lysate
Lane 5: HELA Cell Lysate
Lane 6: JURKAT Cell Lysate
Anti-SHC antibody, ASA-B1685, IHC(P)
IHC(P): Rat Brain Tissue
IHC(P): Rat Brain Tissue