More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| serine/arginine-rich splicing factor 1 | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | IHC-P, WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the N-terminus of Human SF2(6-33aa VIRGPAGNNDCRIYVGNLPPDIRTKDIE), identical to the related mouse sequence. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | SRSF1 |
Protein | Serine/arginine-rich splicing factor 1 |
Uniprot ID | Q07955 |
Function | Plays a role in preventing exon skipping, ensuring the accuracy of splicing and regulating alternative splicing. Interacts with other spliceosomal components, via the RS domains, to form a bridge between the 5'- and 3'-splice site binding components, U1 snRNP and U2AF. Can stimulate binding of U1 snRNP to a 5'-splice site-containing pre-mRNA. Binds to purine-rich RNA sequences, either the octamer, 5'-RGAAGAAC-3' (r: A or G) or the decamers, AGGACAGAGC/AGGACGAAGC. Binds preferentially to the 5'- CGAGGCG-3' motif in vitro. Three copies of the octamer constitute a powerful splicing enhancer in vitro, the ASF/SF2 splicing enhancer (ASE) which can specifically activate ASE-dependent splicing. Isoform ASF-2 and isoform ASF-3 act as splicing repressors. May function as export adapter involved in mRNA nuclear export through the TAP/NXF1 pathway. |
Tissue Specificity | |
Sub-cellular localization | Cytoplasm. Nucleus speckle. Note: In nuclear speckles. Shuttles between the nucleus and the cytoplasm. |
Sequence Similarities | Belongs to the splicing factor SR family. |
Aliases | Alternative splicing factor 1 antibody|Alternative-splicing factor 1 antibody| arginine/serine-rich 1 antibody|ASF 1 antibody|ASF antibody|ASF-1 antibody|ASF1 antibody|FLJ53078 antibody|MGC5228 antibody|P33 subunit antibody|Pre mRNA splicing factor SF2 P33 subunit antibody|pre-mRNA-splicing factor SF2 antibody| Serine/arginine-rich splicing factor 1 antibody|SF2 antibody|SF2P33 antibody| SFRS1 antibody|Splicing factor 2 alternate splicing factor antibody|Splicing factor 2 antibody|Splicing factor antibody|Splicing factor arginine/serine rich 1 antibody| SR Splicing factor 1 antibody|SRp30a antibody|srsf1 antibody|SRSF1_HUMAN antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Mouse, Rat | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human, Mouse, Rat | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti- SF2 antibody, ASA-B1674, Western blotting
All lanes: Anti SF2 (ASA-B1674) at 0.5ug/ml
Lane 1: Rat Testis Tissue Lysate at 50ug
Lane 2: Rat Brain Tissue Lysate at 50ug
Lane 3: NEURO Whole Cell Lysate at 40ug
Lane 4: 293T Whole Cell Lysate at 40ug
Lane 5: SW620 Whole Cell Lysate at 40ug
Predicted bind size: 28KD
Observed bind size: 28KD
All lanes: Anti SF2 (ASA-B1674) at 0.5ug/ml
Lane 1: Rat Testis Tissue Lysate at 50ug
Lane 2: Rat Brain Tissue Lysate at 50ug
Lane 3: NEURO Whole Cell Lysate at 40ug
Lane 4: 293T Whole Cell Lysate at 40ug
Lane 5: SW620 Whole Cell Lysate at 40ug
Predicted bind size: 28KD
Observed bind size: 28KD
Anti- SF2 antibody, ASA-B1674, IHC(P)
IHC(P): Mouse Brain Tissue
IHC(P): Mouse Brain Tissue
Anti- SF2 antibody, ASA-B1674, IHC(P)
IHC(P): Rat Brain Tissue
IHC(P): Rat Brain Tissue