More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 1 | Polyclonal | IgG | Rabbit | Mouse, Rat | IHC-P, WB | Immunogen affinity purified. |
Immunogen | ||||||
| E.coli-derived rat SERPINA1 recombinant protein (Position: K211-R411). Rat SERPINA1 shares 66% amino acid (aa) sequence identity with human SERPINA1. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | SERPINA1 |
Protein | Alpha-1-antiproteinase |
Uniprot ID | P17475 |
Function | Inhibitor of serine proteases. The primary target is elastase, but also has a moderate affinity for plasmin and thrombin. |
Tissue Specificity | Plasma. |
Sub-cellular localization | Secreted. |
Sequence Similarities | |
Aliases | A1A antibody|A1AT antibody|A1AT_HUMAN antibody|AAT antibody|Alpha 1 antiproteinase antibody|Alpha 1 antitrypsin antibody|Alpha 1 antitrypsin null antibody|Alpha 1 protease inhibitor antibody|Alpha-1 protease inhibitor antibody|Alpha-1-antiproteinase antibody|alpha-1-AT antibody|alpha1 PI antibody|alpha1 proteinase inhibitor antibody|Alpha1AT antibody|Clade A (alpha 1 antiproteinase antitrypsin) member 1 antibody|Dom1 antibody|MGC23330 antibody|MGC9222 antibody|PI antibody|PI1 antibody|PRO2275 antibody|Protease inhibitor 1 (anti elastase) antibody|Serine (or cysteine) proteinase inhibitor clade A member 1 antibody|Serine protease inhibitor 1-1 antibody|Serine protease inhibitor A1a antibody|Serpin A1 antibody|Serpin A1a antibody|Serpin peptidase inhibitor clade A (alpha 1 antiproteinase antitrypsin) member 1 antibody|Serpin peptidase inhibitor clade A member 1 antibody|SerpinA1 antibody|Short peptide from AAT antibody|SPAAT antibody|Spi1-1 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Mouse, Rat | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Mouse, Rat | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti- SERPINA1 antibody, ASA-B1670, Western blotting
All lanes: Anti SERPINA1 (ASA-B1670) at 0.5ug/ml
Lane 1: Rat Testis Tissue Lysate at 50ug
Lane 2: Mouse Testis Tissue Lysate at 50ug
Lane 3: Rat Liver Tissue Lysate at 50ug
Lane 4: Mouse Liver Tissue Lysate at 50ug
Predicted bind size: 47KD
Observed bind size: 47KD
All lanes: Anti SERPINA1 (ASA-B1670) at 0.5ug/ml
Lane 1: Rat Testis Tissue Lysate at 50ug
Lane 2: Mouse Testis Tissue Lysate at 50ug
Lane 3: Rat Liver Tissue Lysate at 50ug
Lane 4: Mouse Liver Tissue Lysate at 50ug
Predicted bind size: 47KD
Observed bind size: 47KD
Anti- SERPINA1 antibody, ASA-B1670, IHC(P)
IHC(P): Rat Spleen Tissue
IHC(P): Rat Spleen Tissue
Anti- SERPINA1 antibody, ASA-B1670, IHC(P)
IHC(P): Mouse Spleen Tissue
IHC(P): Mouse Spleen Tissue