More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| secretogranin III | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | IHC-P, IHC-F, ICC, WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of human Secretogranin 3(438-450aa DFINKQADAYVEK), different from the related mouse and rat sequences by one amino acid. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | SCG3 |
Protein | Secretogranin-3 |
Uniprot ID | Q8WXD2 |
Function | |
Tissue Specificity | Expressed in brain, heart, kidney, liver and skeletal muscle. |
Sub-cellular localization | Cytoplasmic vesicle, secretory vesicle lumen . Cytoplasmic vesicle, secretory vesicle membrane ; Peripheral membrane protein . Secreted. Note: Associated with the secretory granule membrane through direct binding to cholesterol-enriched lipid rafts in intragranular conditions (By similarity). Neuroendocrine and endocrine secretory granules. |
Sequence Similarities | |
Aliases | 1B1075 antibody|AI385542 antibody|Chgd antibody|SCG3 antibody|SCG3_HUMAN antibody|Secretogranin III antibody|Secretogranin-3 antibody|SGCG antibody|SgIII antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Rat Mouse | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human, Rat Mouse | AssaySolutio's IHC/ICC Detection kit |
| Immunohistochemistry(Frozen Section) | 0.5-1μg/ml | Human, Rat Mouse | AssaySolutio's IHC/ICC Detection kit |
| Immunocytochemistry | 0.5-1μg/ml | Human Mouse | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P) IHC(F) and ICC. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-Secretogranin 3 antibody, ASA-B1661, Western blotting
WB: HELA Cell Lysate
WB: HELA Cell Lysate
Anti-Secretogranin 3 antibody, ASA-B1661, IHC(P)
IHC(P): Rat Brain Tissue
IHC(P): Rat Brain Tissue
Anti-Secretogranin 3 antibody, ASA-B1661, IHC(P)
IHC(P): Human Rectal Cancer Tissue
IHC(P): Human Rectal Cancer Tissue