More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| succinate dehydrogenase complex, subunit B, iron sulfur(Ip) | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | IHC-P, WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the N-terminus of human SDHB(42-59 FAIYRWDPDKAGDKPHMQ), different from the related rat sequence by one amino acid and mouse sequence by two amino acids. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | SDHB |
Protein | Succinate dehydrogenase[ubiquinone] iron-sulfur subunit, mitochondrial |
Uniprot ID | P21912 |
Function | Iron-sulfur protein (IP) subunit of succinate dehydrogenase (SDH) that is involved in complex II of the mitochondrial electron transport chain and is responsible for transferring electrons from succinate to ubiquinone (coenzyme Q). |
Tissue Specificity | |
Sub-cellular localization | Mitochondrion inner membrane; Peripheral membrane protein; Matrix side. |
Sequence Similarities | Belongs to the succinate dehydrogenase/fumarate reductase iron-sulfur protein family. |
Aliases | CWS2 antibody|DHSB_HUMAN antibody|FLJ92337 antibody|IP antibody|Iron sulfur subunit antibody|Iron sulfur subunit of complex II antibody|Iron-sulfur subunit of complex II antibody|mitochondrial antibody|OTTHUMP00000044624 antibody|PGL 4 antibody|PGL4 antibody|SDH 1 antibody|SDH antibody|SDH1 antibody|SDH2 antibody|SDHB antibody|SDHIP antibody|Succinate dehydrogenase[ubiquinone] iron sulfur protein mitochondrial antibody|Succinate dehydrogenase[ubiquinone] iron-sulfur subunit antibody|succinate dehydrogenase[ubiquinone] iron-sulfur subunit, mitochondrial antibody|Succinate Dehydrogenase 1 Iron Sulfur Subunit antibody|Succinate dehydrogenase complex subunit B iron sulfur antibody|Succinate Dehydrogenase Complex Subunit B Iron Sulfur Protein antibody|succinate dehydrogenase complex, subunit B, iron sulfur(Ip) antibody|Succinate dehydrogenase iron sulfur protein antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Rat Mouse | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human, Rat Mouse | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti-SDHB antibody, ASA-B1659, IHC(P)
IHC(P): Rat Cardiac Muscle Tissue
IHC(P): Rat Cardiac Muscle Tissue
Anti-SDHB antibody, ASA-B1659, Western blotting
All lanes: Anti SDHB (ASA-B1659) at 0.5ug/ml
Lane 1: Rat Testis Tissue Lysate at 50ug
Lane 2: Rat Cardiac Muscle Tissue Lysate at 50ug
Lane 3: Jurkat Whole Cell Lysate at 40ug
Predicted bind size: 32KD
Observed bind size: 32KD
All lanes: Anti SDHB (ASA-B1659) at 0.5ug/ml
Lane 1: Rat Testis Tissue Lysate at 50ug
Lane 2: Rat Cardiac Muscle Tissue Lysate at 50ug
Lane 3: Jurkat Whole Cell Lysate at 40ug
Predicted bind size: 32KD
Observed bind size: 32KD
