More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| succinate dehydrogenase complex, subunit A, flavoprotein(Fp) | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | IHC-P, WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of human SDHA(641-656aa YRPVIDKTLNEADCAT), identical to the related rat and mouse sequences. | ||||||
Properties
Form | Liquid |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 50% glycerol, 0.9 mg NaCl, 0.2 mg Na2HPO4. *carrier free antibody available upon request. |
Concentration | |
Storage | At -20 °C for 12 months, as supplied, at 2-8 °C for one month. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | SDHA |
Protein | Succinate dehydrogenase[ubiquinone] flavoprotein subunit, mitochondrial |
Uniprot ID | P31040 |
Function | Flavoprotein (FP) subunit of succinate dehydrogenase (SDH) that is involved in complex II of the mitochondrial electron transport chain and is responsible for transferring electrons from succinate to ubiquinone (coenzyme Q). Can act as a tumor suppressor. |
Tissue Specificity | |
Sub-cellular localization | Mitochondrion inner membrane; Peripheral membrane protein; Matrix side. |
Sequence Similarities | Belongs to the FAD-dependent oxidoreductase 2 family. FRD/SDH subfamily. |
Aliases | CMD1GG antibody|DHSA_HUMAN antibody|Flavoprotein subunit of complex II antibody|FP antibody|PGL5 antibody|SDH 2 antibody|SDH1 antibody|SDH2 antibody|SDHA antibody|SDHF antibody|Succinate dehydrogenase[ubiquinone] flavoprotein subunit antibody|Succinate dehydrogenase[ubiquinone] flavoprotein subunit mitochondrial antibody|Succinate dehydrogenase[ubiquinone] flavoprotein subunit, mitochondrial antibody|Succinate dehydrogenase complex flavoprotein subunit antibody|Succinate dehydrogenase complex flavoprotein subunit precursor antibody|Succinate dehydrogenase complex subunit A antibody|Succinate dehydrogenase complex subunit A flavoprotein antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Rat Mouse | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Rat Mouse | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti-SDHA antibody, ASA-B1656, Western blotting
Lane 1: MCF-7 Cell Lysate
Lane 2: HELA Cell Lysate
Lane 3: JURKAT Cell Lysate
Lane 4: HT1080 Cell Lysate
Lane 5: COLO320 Cell Lysate
Lane 1: MCF-7 Cell Lysate
Lane 2: HELA Cell Lysate
Lane 3: JURKAT Cell Lysate
Lane 4: HT1080 Cell Lysate
Lane 5: COLO320 Cell Lysate
Anti-SDHA antibody, ASA-B1656, IHC(P)
IHC(P): Rat Liver Tissue
IHC(P): Rat Liver Tissue
Anti-SDHA antibody, ASA-B1656, IHC(P)
IHC(P): Human Mammary Cancer Tissue
IHC(P): Human Mammary Cancer Tissue