More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| discs, large homolog 1 (Drosophila) | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | IHC-P, WB | Immunogen affinity purified. |
Immunogen | ||||||
| E.coli-derived human SAP97 recombinant protein (Position: M1-A165). Human SAP97 shares 89.7% and 84.2% amino acid (aa) sequence identity with mouse and rat SAP97, respectively. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | DLG1 |
Protein | Disks large homolog 1 |
Uniprot ID | Q12959 |
Function | Essential multidomain scaffolding protein required for normal development (By similarity). Recruits channels, receptors and signaling molecules to discrete plasma membrane domains in polarized cells. May play a role in adherens junction assembly, signal transduction, cell proliferation, synaptogenesis and lymphocyte activation. Regulates the excitability of cardiac myocytes by modulating the functional expression of Kv4 channels. Functional regulator of Kv1.5 channel. |
Tissue Specificity | Abundantly expressed in atrial myocardium (at protein level). Expressed in lung fibroblasts, cervical epithelial and B-cells (at protein level). Widely expressed, with isoforms displaying different expression profiles. |
Sub-cellular localization | Membrane ; Peripheral membrane protein . Basolateral cell membrane . Endoplasmic reticulum membrane . Cell junction, synapse, postsynaptic cell membrane, postsynaptic density . Cell junction, synapse. Cell membrane, sarcolemma. Note: Colocalizes with EPB41 at regions of intercellular contacts. Basolateral in epithelial cells. May also associate with endoplasmic reticulum membranes. Mainly found in neurons soma, moderately found at postsynaptic densities (By similarity). |
Sequence Similarities | Belongs to the MAGUK family. |
Aliases | Discs large homolog 1 antibody|discs large, Drosophila, homolog of, 1 antibody| discs, large homolog 1 (Drosophila) antibody|Disks large homolog 1 antibody| dJ1061C18.1.1 antibody| DKFZp761P0818 antibody|DKFZp781B0426 antibody| DLG 1 antibody|DLG1 antibody|DLG1_HUMAN antibody|DLGH 1 antibody|DLGH1 antibody|HDLG antibody|Presynaptic protein SAP97 antibody| SAP 97 antibody| SAP-97 antibody|SAP97 antibody|Synapse associated protein 97 antibody| Synapse-associated protein 97 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Mouse, Rat | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti- SAP97 antibody, ASA-B1648, Western blotting
All lanes: Anti SAP97 (ASA-B1648) at 0.5ug/ml
Lane 1: Rat Lung Tissue Lysate at 50ug
Lane 2: Mouse Lung Tissue Lysate at 50ug
Lane 3: HELA Whole Cell Lysate at 40ug
Lane 4: MM231 Whole Cell Lysate at 40ug
Lane 5: COLO320 Whole Cell Lysate at 40ug
Lane 6: A549 Whole Cell Lysate at 40ug
Lane 7: NIH3T3 Whole Cell Lysate at 40ug
Predicted bind size: 130KD
Observed bind size: 130KD
All lanes: Anti SAP97 (ASA-B1648) at 0.5ug/ml
Lane 1: Rat Lung Tissue Lysate at 50ug
Lane 2: Mouse Lung Tissue Lysate at 50ug
Lane 3: HELA Whole Cell Lysate at 40ug
Lane 4: MM231 Whole Cell Lysate at 40ug
Lane 5: COLO320 Whole Cell Lysate at 40ug
Lane 6: A549 Whole Cell Lysate at 40ug
Lane 7: NIH3T3 Whole Cell Lysate at 40ug
Predicted bind size: 130KD
Observed bind size: 130KD
Anti- SAP97 antibody, ASA-B1648, IHC(P)
IHC(P): Human Prostatic Cancer Tissue
IHC(P): Human Prostatic Cancer Tissue
