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Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| runt-related transcription factor 3 | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | IHC-P, WB | Immunogen affinity purified. |
Immunogen | ||||||
| E.coli-derived human RUNX3 recombinant protein (Position: M128-Y270). Human RUNX3 shares 93% amino acid (aa) sequence identity with mouse RUNX3. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | RUNX3 |
Protein | Runt-related transcription factor 3 |
Uniprot ID | Q13761 |
Function | CBF binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, lck, IL-3 and GM-CSF promoters. |
Tissue Specificity | |
Sub-cellular localization | Nucleus. |
Sequence Similarities | |
Aliases | Acute myeloid leukemia 2 protein antibody|Acute myeloid leukemia gene 2 antibody|AML 2 antibody|AML2 antibody|CBF alpha 3 antibody|CBF-alpha-3 antibody|CBFA 3 antibody|CBFA3 antibody|Core binding factor alpha 3 subunit antibody|core binding factor antibody|Core binding factor runt domain alpha subunit 3 antibody|Core binding factor subunit alpha 3 antibody|core-binding factor antibody|Core-binding factor subunit alpha-3 antibody|FLJ34510 antibody|MGC16070 antibody|Oncogene AML 2 antibody|Oncogene AML-2 antibody|PEA2 alpha C antibody|PEA2-alpha C antibody|PEBP2 alpha C antibody|PEBP2-alpha C antibody|Pebp2a3 antibody|PEBP2aC antibody| Polyomavirus enhancer binding protein 2 alpha C subunit antibody| Polyomavirus enhancer-binding protein 2 alpha C subunit antibody|runt domain alpha subunit 3 antibody|runt related transcription factor 3 antibody| Runt-related transcription factor 3 antibody|RUNX 3 antibody|Runx3 antibody| RUNX3_HUMAN antibody|SL3 3 enhancer factor 1 alpha C subunit antibody| SL3-3 enhancer factor 1 alpha C subunit antibody|SL3/AKV core binding factor alpha C subunit antibody|SL3/AKV core-binding factor alpha C subunit antibody|Transcription factor AML2 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human, Mouse, Rat | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti- RUNX3 antibody, ASA-B1642, Western blotting
All lanes: Anti RUNX3 (ASA-B1642) at 0.5ug/ml
Lane 1: A431 Whole Cell Lysate at 40ug
Lane 2: U20S Whole Cell Lysate at 40ug
Predicted bind size: 44KD
Observed bind size: 44KD
All lanes: Anti RUNX3 (ASA-B1642) at 0.5ug/ml
Lane 1: A431 Whole Cell Lysate at 40ug
Lane 2: U20S Whole Cell Lysate at 40ug
Predicted bind size: 44KD
Observed bind size: 44KD
Anti- RUNX3 antibody, ASA-B1642, IHC(P)
IHC(P): Mouse Spleen Tissue
IHC(P): Mouse Spleen Tissue
Anti- RUNX3 antibody, ASA-B1642, IHC(P)
IHC(P): Rat Spleen Tissue
IHC(P): Rat Spleen Tissue