RUNX3 Antibody

Overview

Long Name	Antibody Type	Antibody Isotype	Host	Species Reactivity	Validated Applications	Purification
runt-related transcription factor 3	Polyclonal	IgG	Rabbit	Human, Mouse, Rat	IHC-P, WB	Immunogen affinity purified.
Immunogen
E.coli-derived human RUNX3 recombinant protein (Position: M128-Y270). Human RUNX3 shares 93% amino acid (aa) sequence identity with mouse RUNX3.

Properties

Form	Lyophilized
Size	100 µg/vial
Contents	Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request.
Concentration	Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration).
Storage	At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing.

Additional Information Regarding the Antigen

Gene	RUNX3
Protein	Runt-related transcription factor 3
Uniprot ID	Q13761
Function	CBF binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, lck, IL-3 and GM-CSF promoters.
Tissue Specificity
Sub-cellular localization	Nucleus.
Sequence Similarities
Aliases	Acute myeloid leukemia 2 protein antibody|Acute myeloid leukemia gene 2 antibody|AML 2 antibody|AML2 antibody|CBF alpha 3 antibody|CBF-alpha-3 antibody|CBFA 3 antibody|CBFA3 antibody|Core binding factor alpha 3 subunit antibody|core binding factor antibody|Core binding factor runt domain alpha subunit 3 antibody|Core binding factor subunit alpha 3 antibody|core-binding factor antibody|Core-binding factor subunit alpha-3 antibody|FLJ34510 antibody|MGC16070 antibody|Oncogene AML 2 antibody|Oncogene AML-2 antibody|PEA2 alpha C antibody|PEA2-alpha C antibody|PEBP2 alpha C antibody|PEBP2-alpha C antibody|Pebp2a3 antibody|PEBP2aC antibody| Polyomavirus enhancer binding protein 2 alpha C subunit antibody| Polyomavirus enhancer-binding protein 2 alpha C subunit antibody|runt domain alpha subunit 3 antibody|runt related transcription factor 3 antibody| Runt-related transcription factor 3 antibody|RUNX 3 antibody|Runx3 antibody| RUNX3_HUMAN antibody|SL3 3 enhancer factor 1 alpha C subunit antibody| SL3-3 enhancer factor 1 alpha C subunit antibody|SL3/AKV core binding factor alpha C subunit antibody|SL3/AKV core-binding factor alpha C subunit antibody|Transcription factor AML2 antibody

Application Details

Application	Concentration*	Species	Validated Using**
Western blot	0.1-0.5μg/ml	Human	AssaySolutio's ECL kit
Immunohistochemistry(Paraffin-embedded Section)	0.5-1μg/ml	Human, Mouse, Rat	AssaySolutio's IHC/ICC Detection kit

AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information