More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| receptor tyrosine kinase-like orphan receptor 1 | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | IHC-P, WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of human ROR1(904-918aa NKSQKPYKIDSKQAS), different from the related rat and mouse sequences by one amino acid. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | ROR1 |
Protein | Tyrosine-protein kinase transmembrane receptor ROR1 |
Uniprot ID | Q01973 |
Function | Tyrosine-protein kinase receptor whose role is not yet clear. |
Tissue Specificity | Expressed strongly in human heart, lung and kidney, but weakly in the CNS. Isoform Short is strongly expressed in fetal and adult CNS and in a variety of human cancers, including those originating from CNS or PNS neuroectoderm. |
Sub-cellular localization | Membrane; Single-pass type I membrane protein. |
Sequence Similarities | Belongs to the protein kinase superfamily. Tyr protein kinase family. ROR subfamily. |
Aliases | dJ537F10.1 antibody|MGC99659 antibody|Neurotrophic tyrosine kinase antibody|Neurotrophic tyrosine kinase receptor related 1 antibody|Neurotrophic tyrosine kinase, receptor related 1 antibody|NTRKR1 antibody|OTTHUMP00000010573 antibody|OTTHUMP00000010574 antibody|OTTMUSP00000008344 antibody|Receptor tyrosine kinase like orphan receptor 1 antibody|receptor-related 1 antibody|RGD1559469 antibody|ROR 1 antibody|ROR1 antibody|ROR1_HUMAN antibody|RP11 24J23.1 antibody|Ttyrosine protein kinase transmembrane receptor ROR1 antibody|Tyrosine kinase like orphan receptor 1 antibody|Tyrosine protein kinase transmembrane receptor ROR1 antibody|Tyrosine-protein kinase transmembrane receptor ROR1 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Rat Human, Mouse | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human Mouse, Rat | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti-ROR1 antibody, ASA-B1635, IHC(P)
IHC(P): Human Intestinal Cancer Tissue
IHC(P): Human Intestinal Cancer Tissue
Anti-ROR1 antibody, ASA-B1635, Western Blotting
All lanes: Anti ROR1 (ASA-B1635) at 0.5ug/ml
Lane 1: A549 Whole Cell Lysate at 40ug
Lane 2: HEPG2 Whole Cell Lysate at 40ug
Lane 3: 22RV1 Whole Cell Lysate at 40ug
Predicted bind size: 104KD
Observed bind size: 81KD
All lanes: Anti ROR1 (ASA-B1635) at 0.5ug/ml
Lane 1: A549 Whole Cell Lysate at 40ug
Lane 2: HEPG2 Whole Cell Lysate at 40ug
Lane 3: 22RV1 Whole Cell Lysate at 40ug
Predicted bind size: 104KD
Observed bind size: 81KD
