More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| amyloid beta(A4) precursor protein-binding, family B, member 1 interacting protein | Polyclonal | IgG | Rabbit | Human, Rat | IHC-P, IHC-F, ICC, WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of human APBB1IP(647-666aa EQDFMSDLMKALQKKRGNVS), different from the related rat sequence by one amino acid, and from the related mouse sequence by two amino acids. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | APBB1IP |
Protein | Amyloid beta A4 precursor protein-binding family B member 1-interacting protein |
Uniprot ID | Q7Z5R6 |
Function | Appears to function in the signal transduction from Ras activation to actin cytoskeletal remodeling. Suppresses insulin- induced promoter activities through AP1 and SRE. Mediates Rap1- induced adhesion. |
Tissue Specificity | Widely expressed with high expression in thymus, spleen, lymph node, bone marrow and peripheral leukocytes. |
Sub-cellular localization | Cell membrane ; Peripheral membrane protein . Cell projection, lamellipodium . Cell junction, focal adhesion . Cytoplasm, cytoskeleton . Note: Colocalizes with ENA/VASP proteins at lamellipodia tips and focal adhesions, and F- actin at the leading edge. At the membrane surface, associates, via the PH domain, preferentially with the inositol phosphates, PtdIns(5)P and PtdIns(3)P. This binding appears to be necessary for the efficient interaction of the RA domain to Ras-GTPases (By similarity). |
Sequence Similarities | Belongs to the MRL family. |
Aliases | AB1IP antibody|Amyloid beta A4 precursor protein binding family B member 1 interacting protein antibody|Amyloid beta precursor protein binding family B member 1 antibody|APBB1 interacting protein 1 antibody|OTTHUMP00000019344 antibody|PREL 1 antibody|PREL1 antibody|Proline rich EVH1 ligand 1 antibody|Proline rich protein 73 antibody|Rap1 GTP interacting adapter molecule antibody|RAP1 interacting adaptor molecule antibody|RARP 1 antibody|RARP1 antibody|Retinoic acid responsive proline rich protein 1 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Rat | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human, Rat | AssaySolutio's IHC/ICC Detection kit |
| Immunohistochemistry(Frozen Section) | 0.5-1μg/ml | Rat Human | AssaySolutio's IHC/ICC Detection kit |
| Immunocytochemistry | 0.5-1μg/ml | Human Rat | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P) IHC(F) and ICC. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-RIAM antibody, ASA-B1627, Western blotting
Lane 1: Rat Thymus Tissue Lysate
Lane 2: Rat Spleen Tissue Lysate
Lane 3: Rat RAJI Tissue Lysate
Lane 4: HL-60 Cell Lysate
Lane 1: Rat Thymus Tissue Lysate
Lane 2: Rat Spleen Tissue Lysate
Lane 3: Rat RAJI Tissue Lysate
Lane 4: HL-60 Cell Lysate
Anti-RIAM antibody, ASA-B1627, IHC(P)
IHC(P): Human Tonsil Tissue
IHC(P): Human Tonsil Tissue
Anti-RIAM antibody, ASA-B1627, ICC
ICC: JURKAT Cell
ICC: JURKAT Cell