More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| prohibitin 2 | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | IHC-P, ICC, WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence in the middle region of human REA(111-128aa RPNAQELPSMYQRLGLDY), identical to the related rat and mouse sequences. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | PHB2 |
Protein | Prohibitin-2 |
Uniprot ID | Q99623 |
Function | Acts as a mediator of transcriptional repression by nuclear hormone receptors via recruitment of histone deacetylases (By similarity). Functions as an estrogen receptor (ER)-selective coregulator that potentiates the inhibitory activities of antiestrogens and represses the activity of estrogens. Competes with NCOA1 for modulation of ER transcriptional activity. Probably involved in regulating mitochondrial respiration activity and in aging. |
Tissue Specificity | |
Sub-cellular localization | Mitochondrion inner membrane . Cytoplasm . Nucleus . Note: Also cytoplasmic and nuclear. |
Sequence Similarities | |
Aliases | B cell receptor associated protein BAP37 antibody|B-cell associated protein antibody|B-cell receptor-associated protein BAP37 antibody|Bap 37 antibody|BAP antibody|Bap37 antibody|BCAP 37 antibody|BCAP37 antibody|D prohibitin antibody|D-prohibitin antibody|MGC117268 antibody|p22 antibody|PHB 2 antibody|PHB2 antibody|PHB2_HUMAN antibody|PNAS 141 antibody|PNAS141 antibody|Prohibitin 2 antibody|Prohibitin-2 antibody|Prohibitin2 antibody|Repressor of Estrogen Receptor Activity antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Mouse Rat | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human Mouse, Rat | AssaySolutio's IHC/ICC Detection kit |
| Immunocytochemistry | 0.5-1μg/ml | Human Mouse, Rat | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P) and ICC. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-REA antibody, ASA-B1618, Western blotting
Lane 1: PANC Cell Lysate
Lane 2: COLO320 Cell Lysate
Lane 3: U87Cell Lysate
Lane 4: HEPA Cell Lysate
Lane 1: PANC Cell Lysate
Lane 2: COLO320 Cell Lysate
Lane 3: U87Cell Lysate
Lane 4: HEPA Cell Lysate
Anti-REA antibody, ASA-B1618, IHC(P)
IHC(P): Human Lung Cancer Tissue
IHC(P): Human Lung Cancer Tissue
Anti-REA antibody, ASA-B1618, ICC
ICC: A549 Cell
ICC: A549 Cell