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Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| interleukin 10 | Polyclonal | IgG | Rabbit | Rat | ELISA, IHC-P, WB | Immunogen affinity purified. |
Immunogen | ||||||
| E. coli-derived rat IL-10 recombinant protein(Position: S19-N178). | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | IL10 |
Protein | Interleukin-10(IL-10) |
Uniprot ID | P29456 |
Function | Inhibits the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and by helper T-cells. |
Tissue Specificity | |
Sub-cellular localization | Secreted. |
Sequence Similarities | |
Aliases | CSIF antibody|Cytokine Synthesis Inhibitory Factor antibody|GVHDS antibody|IL 10 antibody|IL-10 antibody|IL10 antibody|IL10A antibody|Interleukin 10 antibody|Interleukin-10 antibody|Interleukin10 antibody|MGC126450 antibody|MGC126451 antibody|T-cell growth inhibitory factor antibody|TGIF antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Rat | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Rat | AssaySolutio's IHC/ICC Detection kit |
| ELISA | 0.1-0.5μg/ml | Rat | Sandwich ELISA format |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti-rat IL10 antibody, ASA-B1613, Western blotting
Lane 1: Recombinant Rat IL10 Protein 10ng
Lane 2: Recombinant Rat IL10 Protein 5ng
Lane 3: Recombinant Rat IL10 Protein 2
Lane 1: Recombinant Rat IL10 Protein 10ng
Lane 2: Recombinant Rat IL10 Protein 5ng
Lane 3: Recombinant Rat IL10 Protein 2
