More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| growth hormone 1 | Polyclonal | IgG | Rabbit | Rat | ELISA, Neu, IP, IHC-P, WB | Immunogen affinity purified. |
Immunogen | ||||||
| E. coli-derived rat GH recombinant protein(Position: F27-F216). | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | GH1 |
Protein | Somatotropin |
Uniprot ID | P01244 |
Function | Plays an important role in growth control. Its major role in stimulating body growth is to stimulate the liver and other tissues to secrete IGF-1. It stimulates both the differentiation and proliferation of myoblasts. It also stimulates amino acid uptake and protein synthesis in muscle and other tissues. |
Tissue Specificity | |
Sub-cellular localization | Secreted. |
Sequence Similarities | |
Aliases | gH antibody|GH-N antibody|GH1 antibody|Growth hormone 1 antibody|Growth hormone antibody|hGH-N antibody|IGHD1B antibody|Pituitary growth hormone antibody|Somatotropin antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Rat | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Rat | AssaySolutio's IHC/ICC Detection kit |
| ELISA | 0.1-0.5μg/ml | Rat | Sandwich ELISA format |
| Neutralization | Rat | Rat | |
| Immunoprecipitation | Rat | Rat |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti-rat Growth Hormone antibody, ASA-B1612, Western blotting
Lane 1: Recombinant Rat GH Protein 10ng
Lane 2: Recombinant Rat GH Protein 5ng
Lane 3: Recombinant Rat GH Protein 2
Lane 1: Recombinant Rat GH Protein 10ng
Lane 2: Recombinant Rat GH Protein 5ng
Lane 3: Recombinant Rat GH Protein 2
