More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| RAB8A, member RAS oncogene family | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | IHC-P, WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of human RAB8A(170-183aa KAKMDKKLEGNSPQ), identical to the related mouse and rat sequences. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | RAB8A |
Protein | Ras-related protein Rab-8A |
Uniprot ID | P61006 |
Function | The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. That Rab is involved in polarized vesicular trafficking and neurotransmitter release. Together with RAB11A, RAB3IP, the exocyst complex, PARD3, PRKCI, ANXA2, CDC42 and DNMBP promotes transcytosis of PODXL to the apical membrane initiation sites (AMIS), apical surface formation and lumenogenesis. Together with MYO5B and RAB11A participates in epithelial cell polarization. Plays an important role in ciliogenesis. Together with MICALL2, may also regulate adherens junction assembly. May play a role in insulin-induced transport to the plasma membrane of the glucose transporter GLUT4 and therefore play a role in glucose homeostasis. |
Tissue Specificity | |
Sub-cellular localization | Cell membrane ; Lipid-anchor ; Cytoplasmic side . Golgi apparatus. Recycling endosome membrane. Cell projection, cilium. Cytoplasmic vesicle, phagosome. Cytoplasmic vesicle, phagosome membrane ; Lipid-anchor ; Cytoplasmic side . Note: Colocalizes with OPTN at the Golgi complex and in vesicular structures close to the plasma membrane. In the GDP- bound form, present in the perinuclear region. Shows a polarized distribution to distal regions of cell protrusions in the GTP- bound form. Colocalizes with PARD3, PRKCI, EXOC5, OCLN, PODXL and RAB11A in apical membrane initiation sites (AMIS) during the generation of apical surface and lumenogenesis. Localizes to tubular recycling endosome. Recruited to phagosomes containing S.aureus or M.tuberculosis. |
Sequence Similarities | |
Aliases | AA409338 antibody|MEL antibody|Mel transforming oncogene(derived from cell line NK14) RAB8 homolog antibody|Mel transforming oncogene(derived from cell line NK14) antibody|Mel transforming oncogene(RAB8 homolog) antibody|Mel transforming oncogene antibody|MGC124948 antibody|Oncogene c mel antibody|Oncogene c-mel antibody|RAB8 antibody|RAB8A antibody|RAB8A member RAS oncogene family antibody|Ras associated protein RAB8 antibody|Ras related protein Rab 8A antibody|Ras-related protein Rab-8A antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Mouse, Rat | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human Mouse, Rat | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti-RAB8A antibody, ASA-B1602, IHC(P)
IHC(P): Human Intestinal Cancer Tissue
IHC(P): Human Intestinal Cancer Tissue
Anti-RAB8A antibody, ASA-B1602, IHC(P)
IHC(P): Human Mammary Cancer Tissue
IHC(P): Human Mammary Cancer Tissue
Anti-RAB8A antibody, ASA-B1602, All Western blotting
All lanes: Anti-RAB8A(ASA-B1602) at 0.5ug/ml
Lane 1: Rat Brain Tissue Lysate at 40ug
Lane 2: Mouse Brain Tissue Lysate at 40ug
Lane 3: Human Placenta Tissue Lysate at 40ug
Lane 4: HELA Whole Cell Lysate at 40ug
Lane 5: PC12 Whole Cell Lysate at 40ug
Lane 6: NIH Whole Cell Lysate at 40ug
Lane 7: A431 Whole Cell Lysate at 40ug
Lane 8: 293T Whole Cell Lysate at 40ug
Predicted bind size: 24KD
Observed bin
All lanes: Anti-RAB8A(ASA-B1602) at 0.5ug/ml
Lane 1: Rat Brain Tissue Lysate at 40ug
Lane 2: Mouse Brain Tissue Lysate at 40ug
Lane 3: Human Placenta Tissue Lysate at 40ug
Lane 4: HELA Whole Cell Lysate at 40ug
Lane 5: PC12 Whole Cell Lysate at 40ug
Lane 6: NIH Whole Cell Lysate at 40ug
Lane 7: A431 Whole Cell Lysate at 40ug
Lane 8: 293T Whole Cell Lysate at 40ug
Predicted bind size: 24KD
Observed bin