More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| RAB5A, member RAS oncogene family | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | IHC-P, WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence in the middle region of human Rab5(138-155aa ANKRAVDFQEAQSYADDN), identical to the related rat and mouse sequences. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | RAB5A |
Protein | Ras-related protein Rab-5A |
Uniprot ID | P20339 |
Function | The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. RAB5A is required for the fusion of plasma membranes and early endosomes. Contributes to the regulation of filopodia extension. |
Tissue Specificity | |
Sub-cellular localization | Cell membrane ; Lipid-anchor ; Cytoplasmic side . Early endosome membrane ; Lipid-anchor . Melanosome. Cytoplasmic vesicle . Cell projection, ruffle . Membrane. Cytoplasm, cytosol. Note: Enriched in stage I melanosomes. Alternates between membrane-bound and cytosolic forms. |
Sequence Similarities | |
Aliases | RAB 5 antibody|RAB 5A antibody|RAB5A antibody|RAB5A member RAS oncogene family antibody|RAB5A_HUMAN antibody|RAS associated protein RAB5A antibody|Ras related protein Rab 5A antibody|Ras-related protein Rab-5A antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Mouse, Rat | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human, Rat Mouse | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti-Rab5 antibody, ASA-B1601, Western blotting
Lane 1: Rat Brain Tissue Lysate
Lane 2: Rat Ovary Tissue Lysate
Lane 3: Human Placenta Tissue Lysate
Lane 4: HELA Cell Lysate
Lane 5: 293T Cell Lysate
Lane 6: A375 Cell Lysate
Lane 7: COLO320 Cell Lysate
Lane 8: MM453 Cell Lysate
Lane 1: Rat Brain Tissue Lysate
Lane 2: Rat Ovary Tissue Lysate
Lane 3: Human Placenta Tissue Lysate
Lane 4: HELA Cell Lysate
Lane 5: 293T Cell Lysate
Lane 6: A375 Cell Lysate
Lane 7: COLO320 Cell Lysate
Lane 8: MM453 Cell Lysate
Anti-Rab5 antibody, ASA-B1601, IHC(P)
IHC(P):Rat Brain Tissue
IHC(P):Rat Brain Tissue
