More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| prominin 1 | Polyclonal | IgG | Rabbit | Human | IHC-P, WB | Immunogen affinity purified. |
Immunogen | ||||||
| E.coli-derived human PROM1 recombinant protein (Position: P531-H865). Human PROM1 shares 61% amino acid (aa) sequence identity with mouse PROM1. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | PROM1 |
Protein | Prominin-1 |
Uniprot ID | O43490 |
Function | May play a role in cell differentiation, proliferation and apoptosis (PubMed:24556617). Binds cholesterol in cholesterol- containing plasma membrane microdomains and may play a role in the organization of the apical plasma membrane in epithelial cells. During early retinal development acts as a key regulator of disk morphogenesis. Involved in regulation of MAPK and Akt signaling pathways. In neuroblastoma cells suppresses cell differentiation such as neurite outgrowth in a RET-dependent manner (PubMed:20818439). |
Tissue Specificity | Isoform 1 is selectively expressed on CD34 hematopoietic stem and progenitor cells in adult and fetal bone marrow, fetal liver, cord blood and adult peripheral blood. Isoform 1 is not detected on other blood cells. Isoform 1 is also expressed in a number of non-lymphoid tissues including retina, pancreas, placenta, kidney, liver, lung, brain and heart. Found in saliva within small membrane particles. Isoform 2 is predominantly expressed in fetal liver, skeletal muscle, kidney, and heart as well as adult pancreas, kidney, liver, lung, and placenta. Isoform 2 is highly expressed in fetal liver, low in bone marrow, and barely detectable in peripheral blood. Isoform 2 is expressed on hematopoietic stem cells and in epidermal basal cells (at protein level). Expressed in adult retina by rod and cone photoreceptor cells (at protein level). |
Sub-cellular localization | Apical cell membrane ; Multi- pass membrane protein . Cell projection, microvillus membrane ; Multi-pass membrane protein . Cell projection, cilium, photoreceptor outer segment . Endoplasmic reticulum. Endoplasmic reticulum-Golgi intermediate compartment. Note: Found in extracellular membrane particles in various body fluids such as cerebrospinal fluid, saliva, seminal fluid and urine. |
Sequence Similarities | Belongs to the prominin family. |
Aliases | AC133|Antigen AC133|CD133 antibody|CORD12|hematopoietic stem cell antigen|hProminin|MCDR2|MSTP061 antibody|PROM1|PROM 1|PROM-1|Prominin-1|Prominin 1|Prominin1|Prominin-like protein 1|PROML1 antibody|RP41|STGD4 |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti-PROM1 antibody, ASA-B1579--1.jpg
All lanes: Anti PROM1 (ASA-B1579) at 0.5ug/ml
WB: Recombinant Human PROM1 Protein 0.5ng
Predicted bind size: 40KD
Observed bind size: 40KD
All lanes: Anti PROM1 (ASA-B1579) at 0.5ug/ml
WB: Recombinant Human PROM1 Protein 0.5ng
Predicted bind size: 40KD
Observed bind size: 40KD
Anti-PROM1 antibody, ASA-B1579--2.jpg
All lanes: Anti PROM1 (ASA-B1579) at 0.5ug/ml
WB: MCF-7 Whole Cell Lysate at 40ug
Predicted bind size: 97KD
Observed bind size: 130KD
All lanes: Anti PROM1 (ASA-B1579) at 0.5ug/ml
WB: MCF-7 Whole Cell Lysate at 40ug
Predicted bind size: 97KD
Observed bind size: 130KD
Anti-PROM1 antibody, ASA-B1579--3.jpg
IHC(P): Human Tonsil Tissue
IHC(P): Human Tonsil Tissue