More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| profilin 2 | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | IHC-P, WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of human Profilin 2(125-140aa NKKAYSMAKYLRDSGF), identical to the related mouse and rat sequences. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | PFN2 |
Protein | Profilin-2 |
Uniprot ID | P35080 |
Function | Binds to actin and affects the structure of the cytoskeleton. At high concentrations, profilin prevents the polymerization of actin, whereas it enhances it at low concentrations. By binding to PIP2, it inhibits the formation of IP3 and DG. |
Tissue Specificity | Highly expressed in brain, skeletal muscle and kidney and less strongly in heart, placenta, lung and liver. |
Sub-cellular localization | Cytoplasm, cytoskeleton. |
Sequence Similarities | |
Aliases | D3S1319E antibody|PFL antibody|PFN 2 antibody|PFN2 antibody|PROF2_HUMAN antibody|Profilin II antibody|Profilin-2 antibody|Profilin2 antibody|ProfilinII antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Rat Human, Mouse | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human, Rat Mouse | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti- Profilin2 antibody, ASA-B1569, Western blotting
All lanes: Anti Profilin2 (ASA-B1569) at 0.5ug/ml
WB: Rat Brain Tissue Lysate at 50ug
Predicted bind size: 15KD
Observed bind size: 15KD
All lanes: Anti Profilin2 (ASA-B1569) at 0.5ug/ml
WB: Rat Brain Tissue Lysate at 50ug
Predicted bind size: 15KD
Observed bind size: 15KD
Anti- Profilin2 antibody, ASA-B1569, IHC(P)
IHC(P): Human Tonsil Tissue
IHC(P): Human Tonsil Tissue
Anti- Profilin2 antibody, ASA-B1569, IHC(P)
IHC(P): Human Intestinal Cancer Tissue
IHC(P): Human Intestinal Cancer Tissue