More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| prion protein | Polyclonal | IgG | Rabbit | Mouse, Rat | IHC-P, WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence in the middle region of mouse Prion protein PrP(143-159aa DWEDRYYRENMYRYPNQ), identical to the related rat sequence. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | PRNP |
Protein | Major prion protein(PrP) |
Uniprot ID | P04925 |
Function | May play a role in neuronal development and synaptic plasticity. May be required for neuronal myelin sheath maintenance. May play a role in iron uptake and iron homeostasis. Soluble oligomers are toxic to cultured neuroblastoma cells and induce apoptosis (in vitro) (By similarity). Association with GPC1 (via its heparan sulfate chains) targets PRNP to lipid rafts. Also provides Cu(2+) or ZN(2+) for the ascorbate-mediated GPC1 deaminase degradation of its heparan sulfate side chains. |
Tissue Specificity | Highly expressed in the brain, lung, kidney and heart. Expressed at low levels in the liver and spleen. |
Sub-cellular localization | Cell membrane ; Lipid-anchor, GPI-anchor . Golgi apparatus . Note: Targeted to lipid rafts via association with the heparan sulfate chains of GPC1. Colocates, in the presence of CU(2+), to vesicles in para- and perinuclear regions, where both proteins undergo internalization. Heparin displaces PRNP from lipid rafts and promotes endocytosis. |
Sequence Similarities | |
Aliases | Alternative prion protein; major prion protein antibody|AltPrP antibody|ASCR antibody|atal familial insomnia antibody|CD230 antibody|CD230 antigen antibody|CJD antibody|Creutzfeld Jakob disease antibody|Creutzfeld Jakob Disease Gerstmann Strausler Scheinker Syndrome Fatal Familial Insomnia antibody|Gerstmann-Strausler-Scheinker syndrome antibody|GSS antibody|KURU antibody|Major prion protein antibody|MGC26679 antibody|p27 30 antibody|PRIO_HUMAN antibody|Prion protein antibody|Prion related protein antibody|PRIP antibody|Prni antibody|Prnp antibody|PrP 27-30 antibody|PrP antibody|PrP27 30 antibody|PrP27-30 antibody|PrP33 35C antibody|PrP33-35C antibody|PrPC antibody|PrPSc antibody|Sinc antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Rat Mouse | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Rat Mouse | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti-Prion protein PrP antibody, ASA-B1566, Western blotting
All lanes: Anti Prion protein PrP (ASA-B1566) at 0.5ug/ml
Lane 1: Rat Brain Tissue Lysate at 50ug
Lane 2: Rat Brain Tissue Lysate at 50ug
Predicted bind size: 28KD
Observed bind size: 28KD
All lanes: Anti Prion protein PrP (ASA-B1566) at 0.5ug/ml
Lane 1: Rat Brain Tissue Lysate at 50ug
Lane 2: Rat Brain Tissue Lysate at 50ug
Predicted bind size: 28KD
Observed bind size: 28KD
Anti-Prion protein PrP antibody, ASA-B1566, IHC(P)
IHC(P): Rat Brain Tissue
IHC(P): Rat Brain Tissue
