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Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| peroxisome proliferator-activated receptor alpha | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | IHC-P, IHC-F, ICC, WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of mouse PPAR alpha(393-412aa NIGYIEKLQEGIVHVLKLHL), different from the human sequence by four amino acids. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | PPARA |
Protein | Peroxisome proliferator-activated receptor alpha(PPAR-alpha) |
Uniprot ID | P23204 |
Function | Ligand-activated transcription factor. Key regulator of lipid metabolism. Activated by the endogenous ligand 1-palmitoyl- 2-oleoyl-sn-glycerol-3-phosphocholine (16:0/18:1-GPC). Activated by oleylethanolamide, a naturally occurring lipid that regulates satiety. Receptor for peroxisome proliferators such as hypolipidemic drugs and fatty acids. Regulates the peroxisomal beta-oxidation pathway of fatty acids. Functions as transcription activator for the ACOX1 and P450 genes. Transactivation activity requires heterodimerization with RXRA and is antagonized by NR2C2. May be required for the propagation of clock information to metabolic pathways regulated by PER2. |
Tissue Specificity | Highly expressed in liver, kidney and heart. Very weakly expressed in brain and testis. |
Sub-cellular localization | Nucleus. |
Sequence Similarities | Belongs to the nuclear hormone receptor family. NR1 subfamily. |
Aliases | hPPAR antibody|MGC2237 antibody|MGC2452 antibody|NR1C1 antibody|Nuclear receptor subfamily 1 group C member 1 antibody|OTTHUMP00000197740 antibody|OTTHUMP00000197741 antibody|Peroxisome Proliferator Activated Receptor alpha antibody|Peroxisome proliferator-activated receptor alpha antibody|PPAR antibody|PPAR-alpha antibody|PPARA antibody|PPARA_HUMAN antibody|PPARalpha antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Mouse, Rat | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human, Mouse, Rat | AssaySolutio's IHC/ICC Detection kit |
| Immunohistochemistry(Frozen Section) | 0.5-1μg/ml | Human Mouse, Rat | AssaySolutio's IHC/ICC Detection kit |
| Immunocytochemistry | 0.5-1μg/ml | Human Mouse, Rat | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P) IHC(F) and ICC. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-PPAR alpha antibody, ASA-B1558, Western blotting
Lane 1: Rat Liver Tissue Lysate
Lane 2: Rat Brain Tissue Lysate
Lane 3: Rat Cardiac Muscle Tissue Lysate
Lane 4: MM231 Cell Lysate
Lane 5: HELA Cell Lysate
Lane 6: SMMC Cell Lysate
Lane 7: HT1080 Cell Lysate
Lane 8: SW620 Cell Lysate
Lane 1: Rat Liver Tissue Lysate
Lane 2: Rat Brain Tissue Lysate
Lane 3: Rat Cardiac Muscle Tissue Lysate
Lane 4: MM231 Cell Lysate
Lane 5: HELA Cell Lysate
Lane 6: SMMC Cell Lysate
Lane 7: HT1080 Cell Lysate
Lane 8: SW620 Cell Lysate
Anti-PPAR alpha antibody, ASA-B1558, IHC(P)
IHC(P): Human Mammary Cancer Tissue
IHC(P): Human Mammary Cancer Tissue
Anti-PPAR alpha antibody, ASA-B1558, IHC(P)
IHC(P): Human Mammary Cancer Tissue
IHC(P): Human Mammary Cancer Tissue