PP2A Antibody View larger

PP2A Antibody

ASA-B1557

$330.00

More info

Overview

Long Name

Antibody Type

Antibody Isotype

Host

Species Reactivity

Validated Applications

Purification 

protein phosphatase 2, catalytic subunit, alpha isozyme Polyclonal IgG Rabbit Human, Mouse, Rat IHC-P, WB Immunogen affinity purified.

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminus of human PP2A(6-20aa FTKELDQWIEQLNEC), identical to the related rat and mouse sequences.

Properties

Form

Lyophilized

Size

100 µg/vial

Contents

Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request.

Concentration

Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration).

Storage

At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing.

Additional Information Regarding the Antigen

Gene

PPP2CA

Protein

Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform(PP2A-alpha)

Uniprot ID

P67775

Function

PP2A is the major phosphatase for microtubule-associated proteins (MAPs). PP2A can modulate the activity of phosphorylase B kinase casein kinase 2, mitogen-stimulated S6 kinase, and MAP-2 kinase. Cooperates with SGOL2 to protect centromeric cohesin from separase-mediated cleavage in oocytes specifically during meiosis I (By similarity). Can dephosphorylate SV40 large T antigen and p53/TP53. Activates RAF1 by dephosphorylating it at 'Ser-259'.

Tissue Specificity

Sub-cellular localization

Cytoplasm.

Sequence Similarities

Aliases

PP2A alpha antibody|PP2A-alpha antibody|PP2AA_HUMAN antibody|PP2Aalpha antibody|PP2Ac antibody|PP2CA antibody|PP2Calpha antibody|PPP2CA antibody|Protein phosphatase 2(formerly 2A) catalytic subunit alpha isoform antibody|Protein phosphatase 2 catalytic subunit alpha isoform antibody|Protein phosphatase 2, catalytic subunit, alpha isozyme antibody|Protein phosphatase 2A catalytic subunit, alpha isoform antibody|Replication protein C antibody|RP C antibody|RP-C antibody|RPC antibody|Serine/threonine protein phosphatase 2A catalytic subunit alpha isoform antibody|Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform antibody

Application Details

Application Concentration* Species Validated Using**
Western blot 0.1-0.5μg/ml Human, Rat MouseAssaySolutio's ECL kit
Immunohistochemistry(Paraffin-embedded Section) 0.5-1μg/ml Human, Rat MouseAssaySolutio's IHC/ICC Detection kit

AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti-PP2A antibody, ASA-B1557, Western blotting
All lanes: Anti PP2A(ASA-B1557) at 0.5ug/ml
Lane 1: Rat kidney Tissue Lysate at 50ug
Lane 2: HELA Whole Cell Lysate at 40ug
Predicted bind size: 36KD
Observed bind size: 36KD
Anti-PP2A antibody, ASA-B1557, IHC(P)
IHC(P): Human Mammary Cancer Tissue
Anti-PP2A antibody, ASA-B1557, IHC(P)
IHC(P): Rat Kidney Tissue

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