More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| pleiotrophin | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | IHC-P, WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of human Pleiotrophin(150-168aa QAESKKKKKEGKKQEKMLD), identical to the related rat and mouse sequences. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | PTN |
Protein | Pleiotrophin(PTN) |
Uniprot ID | P21246 |
Function | Secreted growth factor that induces neurite outgrowth and which is mitogenic for fibroblasts, epithelial, and endothelial cells. Binds anaplastic lymphoma kinase (ALK) which induces MAPK pathway activation, an important step in the anti- apoptotic signaling of PTN and regulation of cell proliferation. |
Tissue Specificity | Osteoblast and brain. |
Sub-cellular localization | Secreted. |
Sequence Similarities | Belongs to the pleiotrophin family. |
Aliases | HARP antibody|HB-GAM antibody|HBBM antibody|HBGAM antibody|HBGF-8 antibody|HBGF8 antibody|HBNF antibody|HBNF-1 antibody|HBNF1 antibody|Heparin binding growth associated molecule antibody|Heparin binding growth factor 8 antibody|Heparin binding neurite outgrowth promoting factor 1 antibody|Heparin-binding brain mitogen antibody|Heparin-binding growth factor 8 antibody|Heparin-binding growth-associated molecule antibody|Heparin-binding neurite outgrowth-promoting factor 1 antibody|NEGF1 antibody|Neurite growth promoting factor 1 antibody|OSF-1 antibody|OSF1 antibody|Osteoblast-specific factor 1 antibody|Pleiotrophin antibody|PTN antibody|PTN_HUMAN antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Rat Mouse | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human Mouse, Rat | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti-Pleiotrophin antibody, ASA-B1543, Western blotting
Lane 1: Rat Brain Tissue Lysate
Lane 2: Rat Kidney Tissue Lysate
Lane 3: MCF-7 Cell Lysate
Lane 4: HT1080 Cell Lysate
Lane 5: SMMC Cell Lysate
Lane 1: Rat Brain Tissue Lysate
Lane 2: Rat Kidney Tissue Lysate
Lane 3: MCF-7 Cell Lysate
Lane 4: HT1080 Cell Lysate
Lane 5: SMMC Cell Lysate
Anti-Pleiotrophin antibody, ASA-B1543, IHC(P)
IHC(P): Human Mammary Cancer Tissue
IHC(P): Human Mammary Cancer Tissue
