More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| phosphoinositide-3-kinase, regulatory subunit 2(beta) | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | IHC-P, WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence in the middle region of human PI 3 Kinase p85 beta(447-461aa KVYHQQYQDKSREYD), identical to the related rat and mouse sequences. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | PIK3R2 |
Protein | Phosphatidylinositol 3-kinase regulatory subunit beta(PI3-kinase regulatory subunit beta/PI3K regulatory subunit beta/PtdIns-3-kinase regulatory subunit beta) |
Uniprot ID | O00459 |
Function | Regulatory subunit of phosphoinositide-3-kinase (PI3K), a kinase that phosphorylates PtdIns(4,5)P2 (Phosphatidylinositol 4,5-bisphosphate) to generate phosphatidylinositol 3,4,5- trisphosphate (PIP3). PIP3 plays a key role by recruiting PH domain-containing proteins to the membrane, including AKT1 and PDPK1, activating signaling cascades involved in cell growth, survival, proliferation, motility and morphology. Binds to activated (phosphorylated) protein-tyrosine kinases, through its SH2 domain, and acts as an adapter, mediating the association of the p110 catalytic unit to the plasma membrane. Indirectly regulates autophagy (PubMed:23604317). Promotes nuclear translocation of XBP1 isoform 2 in a ER stress- and/or insulin- dependent manner during metabolic overloading in the liver and hence plays a role in glucose tolerance improvement (By similarity). |
Tissue Specificity | |
Sub-cellular localization | |
Sequence Similarities | Belongs to the PI3K p85 subunit family. |
Aliases | p85 antibody|p85 beta antibody|P85B antibody|P85B_HUMAN antibody|Phosphatidylinositol 3 kinase antibody|Phosphatidylinositol 3 kinase regulatory beta subunit antibody|Phosphatidylinositol 3 kinase regulatory subunit beta antibody|Phosphatidylinositol 3 kinase regulatory subunit polypeptide 2 antibody|Phosphatidylinositol 3 kinase, regulatory subunit, polypeptide 2(p85 beta) antibody|Phosphatidylinositol 3-kinase 85 kDa regulatory subunit beta antibody|Phosphatidylinositol 3-kinase regulatory subunit beta antibody|Phosphoinositide 3 kinase regulatory subunit 2(beta) antibody|Phosphoinositide 3 kinase regulatory subunit 2 antibody|Phosphoinositide 3 kinase regulatory subunit polypeptide 2(p85 beta) antibody|Phosphoinositide 3 kinase regulatory subunit polypeptide 2 antibody|Phosphoinositide 3 kinase, regulatory subunit 2(beta) antibody|Phosphoinositide 3 kinase, regulatory subunit 2(p85 beta) antibody|PI3 kinase p85 beta subunit antibody|PI3 kinase p85 subunit beta antibody|PI3-kinase regulatory subunit beta antibody|PI3-kinase subunit p85-beta antibody|PI3K antibody|PI3K regulatory subunit beta antibody|PIK3R 2 antibody|PIK3R2 antibody|PtdIns 3 kinase p85 beta antibody|PtdIns-3-kinase regulatory subunit beta antibody|PtdIns-3-kinase regulatory subunit p85-beta antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Rat Mouse | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human, Rat Mouse | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti-PI 3 Kinase p85 beta antibody, ASA-B1520, Western blotting
All lanes: Anti PI 3 Kinase p85 beta (ASA-B1520) at 0.5ug/ml
Lane 1: Rat Testis Tissue Lysate at 50ug
Lane 2: 293T Whole Cell Lysate at 40ug
Lane 3: HELA Whole Cell Lysate at 40ug
Predicted bind size: 81KD
Observed bind size: 81KD
All lanes: Anti PI 3 Kinase p85 beta (ASA-B1520) at 0.5ug/ml
Lane 1: Rat Testis Tissue Lysate at 50ug
Lane 2: 293T Whole Cell Lysate at 40ug
Lane 3: HELA Whole Cell Lysate at 40ug
Predicted bind size: 81KD
Observed bind size: 81KD
Anti-PI 3 Kinase p85 beta antibody, ASA-B1520, IHC(P)
IHC(P): Human Mammary Cancer Tissue
IHC(P): Human Mammary Cancer Tissue
