PGK1 Antibody View larger

PGK1 Antibody

ASA-B1515

$330.00

More info

Overview

Long Name

Antibody Type

Antibody Isotype

Host

Species Reactivity

Validated Applications

Purification 

phosphoglycerate kinase 1 Polyclonal IgG Rabbit Human, Mouse, Rat IHC-P, WB Immunogen affinity purified.

Immunogen

A synthetic peptide corresponding to a sequence in the middle region of human PGK1(166-180aa FGTAHRAHSSMVGVN), identical to the related rat and mouse sequences.

Properties

Form

Lyophilized

Size

100 µg/vial

Contents

Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request.

Concentration

Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration).

Storage

At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing.

Additional Information Regarding the Antigen

Gene

PGK1

Protein

Phosphoglycerate kinase 1

Uniprot ID

P00558

Function

In addition to its role as a glycolytic enzyme, it seems that PGK-1 acts as a polymerase alpha cofactor protein (primer recognition protein).

Tissue Specificity

Sub-cellular localization

Cytoplasm.

Sequence Similarities

Belongs to the phosphoglycerate kinase family.

Aliases

Cell migration-inducing gene 10 protein antibody|MGC117307 antibody|MGC8947 antibody|MIG10 antibody|pgk1 antibody|PGK1_HUMAN antibody|PGKA antibody|Phosphoglycerate kinase 1 antibody|Primer recognition protein 2 antibody|PRP 2 antibody

Application Details

Application Concentration* Species Validated Using**
Western blot 0.1-0.5μg/ml Human, Rat MouseAssaySolutio's ECL kit
Immunohistochemistry(Paraffin-embedded Section) 0.5-1μg/ml Human Rat, MouseAssaySolutio's IHC/ICC Detection kit

AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot and ICC. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-PGK1 antibody, ASA-B1515, Western blotting
Lane 1: Rat Liver Tissue Lysate
Lane 2: Rat Brain Tissue Lysate
Lane 3: Rat Lung Tissue Lysate
Lane 4: A431 Cell Lysate
Lane 5: COLO320 Cell Lysate
Lane 6: HELA Cell Lysate
Lane 7: A549 Cell Lysate
Lane 8: JURKAT Cell Lysate
Anti-PGK1 antibody, ASA-B1515, IHC(P)
IHC(P): Human Lung Cancer Tissue

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